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Renal clearances of14C-inulin and polyfructosan in the rat

Effect of increased ureteral pressure

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Summary

Polyfructosan has been used as a substitute for inulin in GFR determinations. However, the validity of this substitution in conditions where renal tubular permeability to other substances, such as mannitol, sucrose and iothalamate, is increased has not been tested. Experiments were performed on 8 rats to compare the clearances of polyfructosan (CPF) and14C-inulin (CIN) during hydropenia, 3% BW saline expansion, elevation of one ureteral catheter by 30 cm, and following return of increased ureteral pressure to the control level. No significant difference between CPF and CIN could be detected except in the kidney subjected to increased ureteral pressure. However, the magnitude of this difference — which may relate to the different molecular weights of the two compounds — was so small that we conclude that the use of CPF, as an index of GFR, is no less reliable than CIN under the conditions tested.

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References

  1. Bank, N., Mutz, B.F., Aynedjian, H.S.: The role of “leakage” of tubular fluid in anuria due to mercury poisoning. J. Clin. Invest.46, 695–704 (1967).

  2. Berglund, F.: Renal clearances of inulin, polyfructosan-S and a polyethylene glycol (PEG 1000) in the rat. Acta Physiol. Scand.64, 238–244 (1965).

  3. Berglund, F., Engberg, A., Persson, E., Ulfendahl, H.: Renal clearances of labelled inulin (inulin-carboxyl-14C, inulin-methoxy-3H) and a polyethylene glycol (PEG 1000) in the rat. Acta Physiol. Scand.76, 458–462 (1969).

  4. Davis, J.M., Schnermann, J., Horster, M.: Micropuncture method for the determination of nephron filtration rate. A recollection study. Pflügers Arch.333, 271–280 (1972).

  5. Führ, J., Kaczmarczyk, J., Krüttgen, C.D.: Eine einfache colorimetrische Methode zur Inulin-bestimmung fur Nieren-clearance-untersuchungen bei Stoffwechselgesunden und Diabetikern. Klin. Wschr.33, 729–730 (1955).

  6. Gutman, Y., Gottschalk, C.W., Lassiter, W.E.: Micropuncture study of inulin absorption in the rat kidney. Science147, 753–754 (1965).

  7. Harth, O.: Ein neues inulinähnliches Polyfructosesaccharid. Klin. Wschr.41, 769–770 (1963).

  8. Lorentz, W.B. Jr., Lassiter, W.E., Gottschalk, C. W.: Renal tubular permeability during increased intrarenal pressure. J. Clin. Invest.51, 484–492 (1972).

  9. Marsh, D., Frasier, C.: Reliability of inulin for determining volume flow in rat renal cortical tubules. Am. J. Physiol.209, 283–286 (1965).

  10. Mertz, D.P., Sarre, H.: Polyfructosan-S: Eine neue inulinartige Substanz zur Bestimmung des Glomerulusfiltrates und des physiologisch aktiven extracellulären Flussigkeitsvolumens beim Menschen. Klin. Wschr.41, 868–872 (1963).

  11. Tanner, G.A., Klose, R.M.: Micropuncture study of inulin reabsorption in Necturus kidney. Am. J. Physiol.211, 1036–1038 (1966).

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Edwards, B.R., Stern, P. Renal clearances of14C-inulin and polyfructosan in the rat. Pflugers Arch. 369, 281–283 (1977). https://doi.org/10.1007/BF00582196

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Keywords

  • inulin clearance
  • polyfructosan clearance
  • glomerular filtration rate
  • increased ureteral pressure