A comparison of the rates of DNA and RNA synthesis of the dorsal gastrula ectoderm being induced to form neural tissue with the uninduced ventral ectoderm has been made for developing embryos ofRana pipiens. There was a higher rate of DNA synthesis per cell in the dorsal ectoderm, but the rates of RNA synthesis per cell in the induced and uninduced ectoderm were similar. The rate of RNA synthesis based on an equivalent amount of total protein was greater for the induced than for the unindueed ectoderm. This is ascribed to the presence of more cells in the induced ectoderm and this is substantiated by the higher DNA/protein ratio for the induced than for the uninduced ectoderm.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Brown, D. D., Caston, J. D.: Biochemistry of amphibian development. I. Ribosome and protein synthesis in early development ofRana pipiens. Develop. Biol.5, 412–434 (1962).
Burton, K.: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J.62, 315–323 (1956).
Flickinger, R. A., Lauth, M. R., Stambrook, P. J.: An inverse relation between the rate of cell division and RNA synthesis per cell in developing frog embryos. J. Embryol. exp. Morph.23, 571–582 (1970).
Freedman, M. L., Stambrook, P. J., Flickinger, R. A.: The absence of labeled RNA on metaphase chromosomes ofTaricha andRana embryos. Exp. Cell Res.47, 640–643 (1967).
Lowry, O. H., Rosebrough, N. J., Farr, H. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–276 (1951).
Niu, M. C., Twitty, V. C.: The differentiation of gastrula ectoderm in medium conditioned by axial mesoderm. Proc. nat. Acad. Sci. (Wash.)39, 985–989 (1953).
Shumway, W.: Stages in the normal development ofRana pipiens. Anat. Rec.78, 139–148 (1940).
This research was supported by grants from the National Institutes of Health (GM 16236-03) and the National Science Foundation (GB 8029).
About this article
Cite this article
Flickinger, R.A. Comparison of DNA and RNA synthesis in dorsal and ventral frog embryo ectoderm during gastrulation. W. Roux' Archiv f. Entwicklungsmechanik 171, 256–258 (1972). https://doi.org/10.1007/BF00582011
- Total Protein
- Developmental Biology
- Equivalent Amount
- Neural Tissue
- Frog Embryo