Pflügers Archiv

, Volume 408, Issue 1, pp 32–37

Measurement by Quin2 of changes of the intracellular calcium concentration in strips of the rabbit ear artery and of the guinea-pig ileum

  • B. Himpens
  • R. Casteels
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology


Ca2+ transient and force development were investigated in smooth muscle strips of the rabbit ear artery and the longitudinal layer of the guinea-pig ileum by using the fluorescent indicator Quin2. Agonists only transiently increased the flourescence intensity despite the enhanced contraction while excess potassium resulted in a maintained light signal. In Ca2+ free solutions the release by an agonist of Ca2+ from an intracellular store can be demonstrated. These observations illustrate the usefullness of the Ca2+ indicator Quin2 in the study of the excitation-contraction coupling in smooth muscle under various conditions.

Key words

Intracellular [Ca2+Smooth muscle Quin2 K+-depolarization Stimulation by agonists 


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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • B. Himpens
    • 1
  • R. Casteels
    • 1
  1. 1.Laboratorium voor FysiologieK.U.L.LeuvenBelgium

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