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  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Effects of some ion transport inhibitors on secretion and reabsorption in intact and perfused single human sweat glands

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Abstract

The effects of three electrolyte transport inhibitors on the secretory and absorptive function of the human eccrine sweat gland were examined. Amiloride and furosemide at 10−4 mol/l had no detectable effect on secretion while ouabain at 10−4 mol/l completely inhibited secretion. In contrast, when amiloride, ouabain and furosemide were applied at 10−4 mol/l to the luminal surface of the sweat duct by microperfusion, only amiloride blocked Na reabsorption. When the same inhibitors were added to the bathing solution at the same concentration, only ouabain inhibited Na reabsorption detectably. Present findings suggest that the secretory epithelium is permeable to solute and water movement while the duct epithelium is probably impermeable with respect to fluid movement across it.

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References

  1. Bentley PJ (1979) The comparative pharmacology of amiloride. In: Cuthbert AW, Fanelli GM, Jr, Scriabine A (eds) Amiloride and epithelial transport. Urban & Schwarzenberg, Baltimore Munich, pp 35–40

  2. Burg MB, Grantham J, Abramow M, Orloff J (1966) Preparation and study of fragments of single rabbit nephrons. Am J Physiol 210:1293–1298

  3. Candia OA (1972) Short-circuit current related to active transport of chloride in frog cornea: effects on chloride fluxes across the isolated bull-frog cornea. Am J Physiol 233:1053–1057

  4. Emrich HM, Ullrich KJ (1966) Ausscheidung verschiedener Stoffe im Schweiß in Abhängigkeit von der Schweißflußrate. Pflügers Arch 290:298–310

  5. Fromter E, Diamond JM (1972) Route of passive ion permeation in epithelia. Nature 235:9–13

  6. Karnaky KJ, Degnan KJ, Zadunaisky JA (1977) Chloride transport across isolated opercular epithelium of killifish: a membrane rich in chloride cells. Science 195:203–205

  7. Mangos J (1973) Transductal fluxes of Na, K, and water in the human eccrine sweat gland. Am J Physiol 224:1235–1240

  8. Prompt CA, Quinton PM (1978) Functions of calcium in sweat secretion. Nature 272:171–172

  9. Quinton PM (1978) Techniques for microdrop analysis of fluids (sweat, saliva, urine) with an energy-dispersive X-ray spectrometer on a scanning electron microscope. Am J Physiol 234:F255-F259

  10. Quinton PM, Tormey JMcD (1976) Localization of Na/K-ATPase sites in the secretory and reabsorptive epithelia of perfused eccrine sweat glands: A question to the role of the enzyme in secretion. J Membr Biol 29:383–399

  11. Sato K (1973) Sweat induction from an isolated eccrine sweat gland. Am J Physiol 225:1147–1152

  12. Sato K (1977) The physiology, pharmacology, and biochemistry of the eccrine sweat gland. In: Rev Physiol, Biochem, Pharmacol Springer, Berlin Heidelberg New York, pp 51–131

  13. Schulz I, Ullrich KJ, Fromter E, Emrich HM, Frick A, Hegel U, Holgrove H (1964) Micropuncture Experiments on Human Sweat Gland. In: Di Sant' Agnese PA (ed) Research on pathogenesis of cystic fibrosis, NIAMD, Bethesda, MD, pp 136–146

  14. Silva P, Stoff J, Field M, Fine L, Forrest JN, Epstein F (1977) Mechanism of active chloride secretion by shark rectal gland: role of Na−K-ATPase in chloride transport. Am J Physiol 233:F298-F306

  15. Ussing HH, Erlij D, Lassen U (1974) Transport pathways in biological membranes. Ann Rev Physiol 36:17–50

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Quinton, P.M. Effects of some ion transport inhibitors on secretion and reabsorption in intact and perfused single human sweat glands. Pflugers Arch. 391, 309–313 (1981). https://doi.org/10.1007/BF00581513

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Key words

  • Amiloride
  • Furosemide
  • Ouabain
  • Microperfusion