Abstract
To compare the activity of Na−K-ATPase in the red blood cells (RBCs) and in renal tissue in disorders of Na+ metabolism, the following groups of rats were studied: 1) control, intact rats, 2) adrenalectomized (ADX) rats, 3) intact rats treated with DOCA, 4) ADX DOCA-treated rats, 5) intact salt-loaded rats, 6) ADX salt-loaded rats, 7) intact dexamethasone-treated rats (DEXA), and 8) ADX DEXA-treated rats. After adrenalectomy (group 2) serum Na1 decreased and serum K+ increased.
Renal Na−K-ATPase in cortex, medulla and papilla of the control group was 44±2.7 μmol Pi/mg prot/h, 128.2±5.9 and 44±3.2 respectively and in group 2 the enzyme activity was 32.5±2.0 (P<0.005), 81.7±4.5 (P<0.001) and 23.6±1.9 (P<0.001) respectively. RBCs Na−K-ATPase of control animals was 2.82±0.19 μmol Pi/mg prot/h, while in group 2 the activity was 1.43±0.24 (P<0.001). DOCA treatment of ADX rats (group 4) normalized serum electrolytes and Na−K-ATPase activity in the renal cortex and papilla and in the RBCs. In the renal medulla the correction by DOCA was only partial. Salt loading of ADX rats (group 6) normalized serum electrolytes and Na−K-ATPase activity in the renal medulla and RBCs. Salt loading of normal rats increased RBC Na−K-ATPase to 3.72±0.36 (P<0.02) and medullary Na−K-ATPase to 185.6±9.8 (P<0.01). DEXA treatment of ADX rats (group 8) corrected only partially the abnormalities in serum electrolytes and Na−K-ATPase activity in the kidney and in the RBCs. These findings show, 1) parallel changes in the activity of Na−K-ATPase in the RBCs and in the kidney after adrenalectomy, 2) parallel changes in the enzyme activity in RBCs and in the kidney medulla after salt loading, and 3) correction towards normal of RBC Na−K-ATPase after ADX by NaCl and DOCA treatment.
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This study was supported by the Morton S. Kaufman Hemodialysis Foundation
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Wald, H., Scherzer, P. & Popovtzer, M.M. Parallel changes in red blood cell and renal Na−K-ATPase activity in adrenal and electrolyte disorders in the rat. Pflugers Arch. 404, 56–60 (1985). https://doi.org/10.1007/BF00581491
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DOI: https://doi.org/10.1007/BF00581491