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  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Water turnover and the measurement of milk intake


Physiologically acceptable methods for estimating milk intake in experimental animals are based on the measurement of rates of disappearance of3H2O from their body-water. This work discusses the problems involved in using methods of this type when steady-states (constant tracee inflow and outflow rates, and constant pool-size) do not exist. Theoretically sound techniques are developed and their use justified.

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  1. Adolph EF, Heggeness FW (1971) Age changes in body water and fat in fetal and infant mammals. Growth 35:55–63

  2. Baverstock P, Green B (1975) Water recycling in lactation. Science 187:657–658

  3. Bergmann KE, Ziegler EE, Fomon SJ (1974) In: SJ Fomon (ed) Infant nutrition. W. B. Saunders Co., Philadelphia London Toronto, pp 245–266

  4. Buss DH, Voss WR (1971) Evaluation of four methods for estimating the milk yields of baboons. J Nutr 101:901–909

  5. Coward WA, Whitehead RG, Sawyer MB, Prentice AM, Evans J (1979) New method for measuring milk intake in breast-fed babies. Lancet II:13–14

  6. Coward WA, Cole TJ, Gerber H (1980) Measurement of milk intake in suckling mice. J Nutr 110:371–372

  7. Fuchs AR (1969) Ethanol and the inhibition of oxytocin release in lactating rats. Acta Endocr 62:546–554

  8. Kametaka M, Inaba J, Ichikawa R (1974) Estimation of the daily milk intake of the suckling rat using the turnover rate of potassium. J Nutr Sci Vitaminol 20:421–429

  9. Kumaresan P, Anderson RR, Turner CW (1967) Effect of litter size upon milk yield and litter weight gain in rats. Proc Soc Exp Biol Med 126:41–45

  10. McBurney JJ, Meier H, Hoag WG (1964) Device for milking mice. J Lab Clin Med 64:485–487

  11. McEwen EH, Whitehead PE (1971) Measurement of the milk intake of reindeer and caribou calves using tritiated water. Canad J Zool 49:443–447

  12. Macfarlane WV, Howard B, Siebert BD (1969) Tritiated water in the measurement of milk intake and tissue growth in ruminants in the field. Nature (Lond) 221:578–579

  13. Moon RC (1969) Mammary growth and milk yield as related to litter size. Proc Soc Exp Biol Med 130:1126–1128

  14. Morag M (1970) Estimation of milk yield in the rat. Lab Anim 4:259–272

  15. Mueller AJ, Cox WM (1946) The effect of changes in diet on the volume and composition of rat milk. J Nutr 31:249–259

  16. Pinson EA (1952) Water exchanges and barriers as studied by the use of hydrogen isotopes. Physiol Rev 32:123–134

  17. Rath EA, Thenen SW (1979) Use of tritiated water for measurement of 24-hour milk intake in suckling lean and genetically obese (ob/ob) mice. J Nutr 109:840–847

  18. Reddy RR, Donker JD (1964) Effect of litter size and number of lactation upon milk yields in Sprague-Dawley rats with observations on rates of gains of young in litters of various sizes. J Dairy Sci 47:1096–1098

  19. Reddy RR, Donker JD (1965) Effects of different intervals between nursing and duration of suckling on rate of milk production in Sprague-Dawley rats in first lactation. J Dairy Sci 48:978–982

  20. Romero JJ, Cañas R, Baldwin RL (1975) A technique for estimating milk production in rats. J Nutr 105:413–420

  21. Searle TW (1970) Body composition in lambs and young sheep and its prediction in vivo from tritiated water space and body weight. J Agric Sci Camb 74:357–362

  22. Spray CMJ, Widdowson EM (1950) The effect of growth and development on the composition of mammals. Br J Nutr 4:332–353

  23. Wright DE, Jones BA, Geenty KG (1974) Measurement of milk consumption in young ruminants using tritiated water. Proc NZ Soc Anim Prod 34:145–150

  24. Yates NG, Macfarlane WV, Ellis R (1971) The estimation of milk intake and growth of beef calves in the field by using tritiated water. Aust J Agric Res 22:291–306

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Correspondence to W. A. Coward.

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Coward, W.A., Cole, T.J., Gerber, H. et al. Water turnover and the measurement of milk intake. Pflugers Arch. 393, 344–347 (1982).

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Key words

  • Milk intake
  • Water turnover
  • Non-steady state