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Pflügers Archiv

, Volume 410, Issue 6, pp 677–678 | Cite as

A simple technique for transferring excised patches of membrane to different solutions for single channel measurements

  • Nino Quartararo
  • Peter H. Barry
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands Short Communication

Abstract

A technical problem associated with the patch clamp technique has been the changing of solutions bathing the membrane patch. The simple technique described here solves this problem by means of a movable polythene sleeve placed on the shaft of the patch clamp pipette. The sleeve is initially placed so that the tip of the pipette is exposed. A gigaohm seal is formed using standard techniques. The patch is then excised and the sleeve is slipped down a few mm past the end of the tip of the pipette. When the pipette and sleeve is now removed from the solution, a small drop of solution covering the membrane patch is held in place at the end of the sleeve by surface tension. The pipette is then easily transferred to a different solution without passing the membrane patch through the air-water interface. The sleeve is then simply pulled back up the pipette shaft to expose the membrane patch to the new solution.

Key words

patch clamp single channels excised patches solution changes 

References

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Nino Quartararo
    • 1
  • Peter H. Barry
    • 1
  1. 1.School of Physiology and PharmacologyUniversity of New South WalesSydneyAustralia

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