The differential toxinogenesis of 25 isolates belonging to species of the potential yeast killer genusPichia that were previously classified in the genusHansenula was comparatively demonstrated by two serologic techniques (indirect immunofluorescence and double immunodiffusion) by using a monoclonal antibody against a yeast killer toxin produced by a selected strain ofPichia anomala (UCSC 25F). The killer phenotypes of thePichia isolates were evaluated by their ability to kill each other. The results, although of insufficient taxonomic value for a reliable separation of either species or genera, attest to the genomic heterogeneity for the killer character in the genusPichia as well as the presumptive dual killer/sensitive identity for each single isolate.
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Polonelli, L., Conti, S., Campani, L. et al. Differential toxinogenesis in the genusPichia detected by an anti-yeast killer toxin monoclonal antibody. Antonie van Leeuwenhoek 59, 139–145 (1991). https://doi.org/10.1007/BF00580653
- killer toxins
- monoclonal antibodies
- Pichia spp.
- serological analysis