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  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Cell Na+ activities and transcellular Na+ absorption by descending colon from normal and Na+-deprived rabbits

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Abstract

The relation between intracellular Na+ activities, (Na)c, determined employing Na+-selective microelectrodes, and the rates of active Na+ absorption,I Na, by rabbit descending colon was examined whenI Na was varied over a wide range by chronic dietary Na+ deprivation. (Na)c averaged 13 mM and was independent ofI Na over a sixfold range. Further, the ratios of the slope resistance of the apical membrane (r m) to that of the basolateral membrane (r s) (i.e.r m/r s) in low-transporters (control diet) and high-transporters (Na+-deprived) did not differ significantly inspite of the fact that the Na+ conductance of the apical membranes of high-transporters was, on the average, three times greater than that of the low-transporters. These findings, together with the results reported by other laboratories, strongly suggest that the aldosterone-induced increase in the conductance of the apical membrane to Na+ and, in turn, the rate of entry of Na+ into the absorptive cells are followed by parallel increases in the ability of cells to extrude Na+ across the basolateral membrane in the absence of a sustained increase in (Na)c as well as the conductance of that barrier.

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Correspondence to Stanley G. Schultz.

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Turnheim, K., Hudson, R.L. & Schultz, S.G. Cell Na+ activities and transcellular Na+ absorption by descending colon from normal and Na+-deprived rabbits. Pflugers Arch. 410, 279–283 (1987). https://doi.org/10.1007/BF00580277

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Key words

  • Rabbit colon
  • Hyperaldosteronism
  • Sodium absorption
  • Cell sodium activity
  • Membrane conductances