Pflügers Archiv

, Volume 354, Issue 2, pp 197–202 | Cite as

A new method for intratubular blockade in micropuncture experiments

  • H. U. Gutsche
  • R. Müller-Suur
  • U. Hegel
  • K. Hierholzer
  • Sieglinde Lüderitz
Short Communications and Technical Notes
Received:

Summary

Criteria for a reliable blockade of tubular flow are generally discussed and experimental evidence is presented that solid paraffin meets all the necessary criteria in rat kidney tubules when an immobile and tight sealing intratubular blockade is requested. A special microinjection apparatus for the application of paraffin is described. Furthermore, several alternative methods and materials have been tested and have been found to be inferior to solid paraffin.

Key words

Micropuncture Intratubular Blockade Paraffin Petroleum Jelly 
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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • H. U. Gutsche
    • 1
  • R. Müller-Suur
    • 1
  • U. Hegel
    • 1
  • K. Hierholzer
    • 1
  • Sieglinde Lüderitz
    • 1
  1. 1.Institut für Klinische PhysiologieKlinikum Steglitz Freie Universität BerlinBerlinGermany

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