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Micropuncture study along the proximal convoluted tubule electrolyte reabsorption in first convolutions

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126 micropunctures were performed on 40 Bowman's capsules as well as along the corresponding proximal convoluted tubules (PCT) on six non-diuretic rats. Capsular and tubular fluid samples (GF and TF) collected were analysed for Na, Cl, K, P, Ca, Mg and3H-Inulin concentrations by electron probe analysis and liquid scintillation. Electrolyte handling along the usually inaccessible part of the PCT, i.e. EPCT, was determined by selecting from the samples collected in the first two or three proximal loops, those having a (TF/GF) In value ≤1.15.

Except for Na the concentration of electrolytes was significantly modified when the fluid flowed along the PCT: 1. In the EPCT, (TF/GF) Cl and K rose and (TF/GF) P dropped in correlation with (TF/GF) In. For (TF/GF) In=1.15, (TF/GF) chloride, potassium and phosphate were respectively equal to 1.12, 1.10 and 0.72. These values were comparable to those obtained in the remaining length of PCT, indicating that the chemical gradient established in the first loops was maintained in the subsequent ones. Therefore proximal handling of these three ions differed depending on the PCT portion considered. The reasons for this difference are discussed further on. 2. Calcium concentration increased and reached a plateau corresponding to (TF/GF)Ca=1.22 for (TF/GF) In values ranging from 1.31 to 1.45. 3. Magnesium was not reabsorbed for (TF/GF) In values below 1.90. Above this level slight Mg reabsorption occurred. 4. Significant correlations between (TF/GF) K, Ca and P and (TF/GF) Cl ratios were observed along the PCT, suggesting that proximal reabsorption of K, Ca and P was dependent on tubular fluid acidification.

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  1. 1.

    Agus, Z. S., Puschett, J. B., Senesky, D., Goldberg, M.: Mode of action of parathyroid hormone and cyclic adenosine 3′,5′-monophosphate on renal tubular phosphate reabsorption in the dog. J. clin. Invest.50, 617 (1971)

  2. 2.

    Amiel, C., Kuntzinger, H., Richet, G.: Micropuncture study of handling of phosphate by proximal and distal nephron in normal and parathyroidectomized rat. Evidence for distal absorption. Pflügers Arch.317, 93 (1970)

  3. 3.

    Bank, N., Aynedjian, H. S.: A micropuncture study of renal bicarbonate and chloride reabsorption in hypokalaemic alkalosis. Clin. Sci.29, 159 (1965)

  4. 4.

    Bloomer, H. A., Rector, F. C., Jr., Seldin, D. W.: The mechanism of potassium reabsorption in the proximal tubule of the rat. J. clin. Invest.42, 277 (1963)

  5. 5.

    Boulpaep, E. L., Seely, J. F.: Electrophysiology of proximal and distal tubules in the autoperfused dog kidney. Amer. J. Physiol.221, 1084 (1971)

  6. 6.

    Bray, G. A.: A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem.1, 279 (1960)

  7. 7.

    Frömter, E., Rumrich, G., Ullrich, K. J.: Phenomenologic description of Na+, Cl and HCO3 absorption from proximal tubu es of the rat kidney. Pflügers Arch.343, 189 (1973)

  8. 8.

    Fulop, M., Brazeau, P.: Phosphaturic effect of sodium bicarbonate and acetazolamide in dogs. J. clin. Invest.47, 983 (1967)

  9. 9.

    Gekle, D., Kossmann, K.: Der Einfluß von Thyreocalcitonin auf die Phosphatreabsorption der Niere (Mikropunktionsuntersuchungen). Mschr. Kinderheilk.116, 308 (1968)

  10. 10.

    Giebisch, G., Klose, R. M., Malnic, G.: Renal tubular potassium transport. Bull. schweiz. Akad. med. Wiss.23, 287 (1967)

  11. 11.

    Gottschalk, C. W., Lassiter, W. E., Mylle, M.: Localization of urine acidification in the mammalian kidney. Amer. J. Physiol.198, 581 (1960)

  12. 12.

    Gottschalk, C. W., Mylle, M.: Micropuncture study of pressures in proximal tubules and peritubular capillaries of the rat kidney and their relation to ureteral and renal venous pressures. Amer. J. Physiol.185, 430 (1956)

  13. 13.

    Kashgarian, M., Warren, Y., Levitin, H.: Micropuncture study of proximal renal tubular chloride transport during hypercapnia in the rat. Amer. J. Physiol.209, 655 (1965)

  14. 14.

    Khuri, R. N., Agulian, S. K., Bogharian, K.: Electrochemical potentials of potassium in proximal renal tubule of rat. Pflügers Arch.346, 319 (1974)

  15. 15.

    Kuntzinger, H., Antonetti, A., Couette, S., Coureau, C., Amiel, C.: Ultramicro (nanoliter range) determination of calcium concentration (10−3 M) by atomic absorption. Analyt. Biochem. (accepted for publication)

  16. 16.

    Lacaz Vieira, F., Malnic, G.: Hydrogen ion secretion by renal cortical tubule as studied by an antimony microelectrode. Amer. J. Physiol.214, 710 (1968)

  17. 17.

    Lassiter, W. E., Gottschalk, C. W., Mylle, M.: Micropuncture study of renal tubular reabsorption of calcium in normal rodents. Amer. J. Physiol.204, 771 (1963)

  18. 18.

    Le Grimellec, Chr., Poujeol, P., Rouffignac, C. de:3H-Inulin and electrolyte concentrations in Bowman's capsule in rat kidney. Comparison with artificial ultrafiltration. Pflügers Arch. (in press)

  19. 19.

    Le Grimellec, Chr., Roinel, N., Morel, F.: Simultaneous Mg, Ca, P, K, Na and Cl analysis in rat tubular fluid. I. During perfusion of either inulin or ferrocyanide. Pflügers Arch.340, 181 (1973)

  20. 20.

    Lingard, J., Rumrich, G., Young, J. A.: Reabsorption of L. Glutamine and L. Histidine from various regions of the rat proximal convolution studied by stationary microperfusion: Evidence that the proximal convolution is not homogeneous. Pflügers Arch.342, 1 (1973)

  21. 21.

    Litchfield, J. B., Bott, P. A.: Micropuncture study of renal excretion of water, K, Na and Cl in the rat. Amer. J. Physiol.203, 667 (1962)

  22. 22.

    Malnic, G., Mello Aires, M., Lacaz Vieira, F.: Chloride excretion in nephrons of rat kidney during alterations of acid-base equilibrium. Amer. J. Physiol.218, 20 (1970)

  23. 23.

    Malvin, R. L., Lotspeich, W. D.: Relation between tubular transport of inorganic phosphate and bicarbonate in the dog. Amer. J. Physiol.187, 51 (1956)

  24. 24.

    Marsh, D. J., Ullrich, K. J., Rumrich, G.: Micropuncture analysis of the behavior of potassium ions in rat renal cortical tubules. Pflügers Arch.277, 107 (1963)

  25. 25.

    Maunsbach, A. B.: Observations on the segmentation of the proximal tubule in the rat kidney. J. Ultrastruct. Res.16, 239 (1966)

  26. 26.

    Morel, F., Roinel, N., Le Grimellec, Chr.: Electron probe analysis of tubular fluid composition. Nephron6, 350 (1969)

  27. 27.

    Murayama, Y., Morel, F., Le Grimellec, Chr.: Phosphate, calcium and magnesium transfers in proximal tubules and loops of Henle, as measured by single nephron microperfusion experiments in the rat. Pflügers Arch.333, 1 (1972)

  28. 28.

    Puschett, J. B., Goldberg, M.: The relationship between the renal handling of phosphate and bicarbornate in man. J. Lab. clin. Med.73, 956 (1969)

  29. 29.

    Ramsay, J. A., Brown, R. H. J.: Simplified apparatus and procedure for freezing point determinations upon small volumes of fluid. J. Sci. Instr.52, 372 (1955)

  30. 30.

    Rector, F. C.: Renal secretion of hydrogen. In the kidney, morphology, biochemistry, Physiology. Rouiller, C. and Müller, A. F., pp. 209–252. Ed. Academic Press 1971

  31. 31.

    Rector, F. C., Carter, N. W., Seldin, D. W.: The mechanism of bicarbonate reabsorption in the proximal and distal tubules of the kidney. J. clin. Invest.44, 278 (1965)

  32. 32.

    Seely, J. F., Boulpaep, E.: Renal function studies on the isobaric autoperfused dog kidney. Amer. J. Physiol.221, 1075 (1971)

  33. 33.

    Strickler, J. C., Thompson, D. D., Klose, R. M., Giebisch, G.: Micropuncture study of inorganic phosphate excretion in the rat. J. clin. Invest.43, 1596 (1964)

  34. 34.

    Terepka, A. R., Chen, P. S., Toribara, T. Y.: Ultrafiltration: a conceptual model and a study of sodium, potassium, chloride and water distribution in normal human sera. Physiol. Chem. Physics.2, 59 (1970)

  35. 35.

    Walker, A. M., Bott, P. A., Oliver, J., MacDowell, M. C.: The collection and analysis of fluid from single nephrons of the mammalian kidney. Amer. J. Physiol.134, 580 (1941)

  36. 36.

    Warren, Y., Luke, R. G., Kashgarian, M., Levitin, H.: Micropuncture studies of chloride and bicarbonate absorption in the proximal renal tubule of the rat in respiratory acidosis and in chloride depletion. Clin. Sci.38, 375 (1970)

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Le Grimellec, C., Giocondi, M.C. & Philippe, P. Micropuncture study along the proximal convoluted tubule electrolyte reabsorption in first convolutions. Pflugers Arch. 354, 133–150 (1975).

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Key words

  • Kidney Tubule
  • Micropuncture
  • Ion Transport
  • Electron Probe Analysis