The effects of BrdU (3×10−4 M) on morphogenesis of the chick embryo explanted at the definitive streak stage and cultured for 24 hours were studied. Compared to controls treated embryos often showed (1) an open neural tube and (2) less numerous somites. Heart development was not significantly affected by BrdU. The damage caused by BrdU was not permanent, i.e., the embryos retained the ability to undergo fairly normal morphogenesis when, after 4–5 hours of BrdU treatment, they were subcultured on a medium with excess thymidine.
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Abbott, J., Holtzer, H.: The loss of phenotypic traits by differentiated cells. V. The effect of 5-bromodeoxyuridine on cloned chondrocytes. Proc. nat. Acad. Sci. (Wash.)59, 1144–1151 (1968)
Billet, F. S., Collini, R., Hamilton, L.: A comparison of the effects of D- and L-threo chloramphenicol on the early development of the chick embryo. J. Embryol. exp. Morph.13, 341–356 (1965)
Bischoff, R., Holtzer, H.: Inhibition of myoblast fusion after one round of DNA synthesis in 5-bromodeoxyuridine. J. Cell. Biol.44, 134–150 (1970)
Bowman, P.: The effect of 2,4-dinitrophenol on the development of early chick embryos. J. Embryol. exp. Morph.17, 425–431 (1967)
Hamburger, V., Hamilton, H. L.: A series of normal stages in the development of the chick embryo. J. Morph.88, 49–92 (1951)
Lee, H., Poprycz, W.: Effects of actinomycin D on explanted early chick embryos. Growth34, 473–454 (1970)
Mazia, D., Gontcharoff, M.: The mitotic behavior of chromosomes in echinoderm eggs following the incorporation of bromodeoxyuridine. Exp. Cell. Res.35, 14–25 (1964)
Mulherkar, L., Rao, K. V., Joshi, S. S.: Studies on some aspects of the role of sulfhydryl groups in morphogenesis. J. Embryol. exp. Morph.14, 129–135 (1965)
New, D. A. T.: A new technique for the cultivation of the chick embryoin vitro. J. Embryol. exp. Morph.3, 326–331 (1955)
Ostertag, W., Crozier, T., Klugen, N., Melderis, H., Dube, S.: Action of 5-bromodeoxyuridine on the induction of haemoglobin synthesis in mouse leukemia cells resistant to 5-BUdR. Nature (Lond.) New Biol.243, 203–205 (1973)
Schubert, D., Jacob, F.: 5-bromodeoxyuridine-induced differentiation of a neuroblastoma. Proc. nat. Acad. Sci. (Wash.)67, 247–254 (1970)
Stellwagen, R. H., Tomkins, G. M.: Preferential inhibition by 5-bromodeoxyuridine of the synthesis of tyrosine aminotransferase in hepatoma cell cultures. J. molec. Biol.56, 167–182 (1971)
Tencer, R., Brachet, J.: Studies on the effects of bromodeoxyuridine (BUdR) on differentiation. Differentiation1, 51–64 (1973)
Wilt, F. H., Anderson, M.: The action of 5-bromodeoxyuridine on differentiation. Develop. Biol.28, 443–447 (1972)
This study was supported by a grant from the Rutgers University Research Council No. 07-2189.
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Lee, H., Deshpande, A.K. & Kalmus, G.W. The effects of 5-bromodeoxyuridine (BrdU) on morphogenesis of the early chick embryo. W. Roux' Archiv f. Entwicklungsmechanik 174, 102–106 (1974). https://doi.org/10.1007/BF00577060
- Developmental Biology
- Neural Tube
- Chick Embryo
- Heart Development