Virus Genes

, Volume 13, Issue 1, pp 87–91 | Cite as

Comparison of nucleic and amino acid sequences and phylogenetic analysis of the GS protein of various equine arteritis virus isolates

  • Nathalie Lepage
  • Gilles St-Laurent
  • Susy Carman
  • Denis Archambault


The genetic variation in equine arteritis virus (EAV) GS protein encoding gene was investigated. Nucleic and deduced amino acid sequences from eight different EAV isolates (one European, two American and five Canadian isolates) were compared with those of the Bucyrus reference strain. Nucleotide and amino acid sequence identities between these isolates and the Bucyrus reference strain ranged from 92.3 to 96.4%, and 93.2 to 95.5%, respectively. However, phylogenetic tree analysis and estimation of genetic distances based on the GS protein encoding gene sequences showed that the European prototype Vienna strain, the American 87AR-A1 isolate and all other North American EAV isolates could be classified into three genetically divergent groups. Our results showed that the GS protein-encoding gene can be subjected on the basis of phylogenetic analysis to genetic variation, as previously shown for the other three EAV structural protein (M, N and GL)-encoding genes.

Key words

equine arteritis virus GS protein genetic variation 


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  1. 1.
    DollE.R., KnappenbergerR.E., and BryansJ.T., Cornell Vet47, 69–75, 1957.Google Scholar
  2. 2.
    TimoneyP.J. and McCollumW.H., Vet Clinics North Amer: Equine Practice9, 295–309, 1993.Google Scholar
  3. 3.
    DenBoonJ.A., SnijderE.J., ChirnsideE.D., DeVriesA.A.F., HorzinekM.C., and SpaanW.J.M., J Virol65, 2910–2920, 1991.Google Scholar
  4. 4.
    SnijderE.J., WassenaarA.L.M., and SpaanW.J.M., J Virol68, 5755–5764, 1994.Google Scholar
  5. 5.
    DeVriesA.A.F., ChirnsideE.D., HorzinekM.C. and RottierP.J.M., J Virol66, 6294–6303, 1992.Google Scholar
  6. 6.
    DeregtD., DeVriesA.A.F., RaamsmanM.J.B., and ElmgrenL.D., J Gen Virol75, 2439–2444, 1994.Google Scholar
  7. 7.
    BalasuryaU.B.R., MacLachlanN.J., DeVriesA.A.F., RossitoP.V. and RottierP.J.M., Virology207, 518–527, 1995.Google Scholar
  8. 8.
    ChirnsideE.D., DeVriesA.A.F., MumfordJ.A., and RottierP.J.M., J Gen Virol76, 1989–1998, 1995.Google Scholar
  9. 9.
    GlaserA.L., DeVriesA.A.F., DuboviE.J., J Gen Virol76, 2223–2233, 1995.Google Scholar
  10. 10.
    DeVriesA.A.F., RaamsmanM.J.B., vanDukH.A., HorzinekM.C., and RottierP.J.M., J Virol69, 3441–3448, 1995.Google Scholar
  11. 11.
    FukunagaY. and McCollumW.H.., Am J Vet Res38, 2043–2046, 1977.Google Scholar
  12. 12.
    FukunagaY., MatsumuraT., SugiuraT., WadaR., ImagawaH., KanemuraT., and KamadaM., Vet Rec134, 574–576, 1994.Google Scholar
  13. 13.
    MurphyT.W., McCollumW.H., TimoneyP.J., KlingebornB.W., HyllsethB., GolnikW., ErasmusB., Vet Microbiol32, 101–115, 1992.Google Scholar
  14. 14.
    ChirnsideE.D., WearningC.M., BinnsM.M., MumfordJ.A., J Gen Virol75, 1491–1497, 1994.Google Scholar
  15. 15.
    BalasuryaU.B.R., TimoneyP.J., McCollumW.H., and MacLachlanN.J., Virology214, 690–697, 1995.Google Scholar
  16. 16.
    St-LaurentG., MorinG., and ArchambaultD., J Clin Microbiol32, 658–665, 1994.Google Scholar
  17. 17.
    ChomczynskiP. and SacchiN., Anal Biochem162, 156–159, 1987.Google Scholar
  18. 18.
    SangerF., NicklenS., and CoulsonA.R., Proc Natl Acad Sci USA74, 5463–5467, 1977.Google Scholar
  19. 19.
    DevreuxJ., HaeberliP., and SmithiesO., Nucleic Acids Res12, 387–395, 1984.Google Scholar
  20. 20.
    FelsensteinJ., PHYLIP (Phylogenic Inference Package) 3.5c Manual, University of Washington, Department of Genetics SK-50, Seattle, WA 98195, 1993.Google Scholar
  21. 21.
    FaabergK.S. and PlagemannP.G.W., Virology212, 512–525, 1995.Google Scholar
  22. 22.
    StraussJ.H. and StraussE.G., Ann Rev Microbiol42, 657–683, 1988.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Nathalie Lepage
    • 1
  • Gilles St-Laurent
    • 1
  • Susy Carman
    • 2
  • Denis Archambault
    • 1
  1. 1.Départment des Sciences BiologiquesUniversité du Québec à MontréalMontréalCanada
  2. 2.Veterinary Laboratory Services BranchOntario Ministry of Agriculture Food and Rural AffairsGuelphCanada

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