Advertisement

Forelimb regeneration from different levels of amputation in the newt,Notophthalmus viridescens: Length, rate, and stages

  • Laurie E. Iten
  • Susan V. Bryant
Article

Summary

  1. 1.

    Some aspects of the influence of position on regeneration have been examined by comparing regeneration from two different levels along the newt forelimb.

     
  2. 2.

    We have defined a series of stages of forelimb regeneration in the newt,Notophthalmus viridescens, in order to facilitate this study.

     
  3. 3.

    Limbs amputated at either a proximal level (through the humerus) or a distal level (through the radius and ulna) pass through the same stages at the same times after amputation.

     
  4. 4.

    The histological sequence of events of digit regeneration was compared with that of limb regeneration from a proximal level of amputation and was found to be the same.

     
  5. 5.

    In limbs amputated at either proximal or distal levels, the rate of elongation of regenerates is the same during the phases of dedifferentiation, blastema accumulation, and blastema growth.

     
  6. 6.

    During the phase of differentiation and morphogenesis, the rate of elongation of regenerates from the proximal level is significantly greater than that of regenerates from the distal level.

     
  7. 7.

    The total length of regenerates from proximal and distal levels along the limb is significantly different.

     
  8. 8.

    The results indicate that positional information does not influence the developmental sequence of events of limb regeneration, but that it does influence the rate of growth of the regenerate and the specification of the structures to be replaced.

     

Keywords

Developmental Biology Positional Information Limb Regeneration Developmental Sequence Distal Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bryant, S. V., Bellairs, A. d'A.: Tail regeneration in the lizardsAnguis fragilis andLacerta dugesii. J. Linn. Soc. Zool.46, 297–305 (1967)Google Scholar
  2. Carlson, B. M.: The histology of inhibition of limb regeneration in the newt,Triturus, by actinomycin D. J. Morph.122, 249–264 (1967)Google Scholar
  3. Chalkley, D. T.: A quantitative histological analysis of forelimb regeneration inTriturus viridescens. J. Morph.94, 21–70 (1954)Google Scholar
  4. Ellis, M. M.: The relation of the amount of tail regenerated to the amount removed in tadpoles ofRana clamitans. J. exp. Zool.7, 421–455 (1909)Google Scholar
  5. Goodwin, P. A.: A comparison of regeneration rates and metamorphosis inTriturus andAmblystoma. Growth10, 75–87 (1946)Google Scholar
  6. Goss, R. J.: Principles of regeneration. New York: Academic Press 1969Google Scholar
  7. Liversage, R. A., Scadding, S. R.: Re-establishment of forelimb regeneration in adult hypophysectomizedDiemictylus (Triturus) viridescens given frog anterior pituitary extract. J. exp. Zool.170, 381–396 (1969)Google Scholar
  8. Maderson, P. F. A., Licht, P.: Factors influencing rates of tail regeneration in the lizardAnolis carolinensis. Experientia (Basel)24, 1083–1086 (1968)Google Scholar
  9. Maderson, P. F. A., Salthe, S. N.: Further observations on tail regeneration inAnolis carolinensis (Iguanidae, Lacertilia). J. exp. Zool.177, 185–189 (1971)Google Scholar
  10. Manner, H. W.: The origin of the blastema and of new tissues in regenerating forelimbs of adultTriturus viridescens virideseens (Rafinesque). J. exp. Zool.122, 229–257 (1953)Google Scholar
  11. Manner, H. W., Zapisek, W. F., Vallee, J. A.: The effect of body size on the rate of salamander limb regeneration. Anat. Rec.137, 378 (1960)Google Scholar
  12. Morgan, T. H.: The physiology of regeneration. J. exp. Zool.3, 457–500 (1906)Google Scholar
  13. Pritchett, W. H., Dent, J. N.: The role of size in the rate of limb regeneration in the adult newt. Growth36, 275–289 (1972)Google Scholar
  14. Rose, S. M.: Epidermal dedifferentiation during blastema formation in the regenerating limbs ofTriturus viridescens. J. exp. Zool.108, 337–361 (1948)Google Scholar
  15. Schauble, M. K.: Seasonal variation of newt forelimb regeneration under controlled environmental conditions. J. exp. Zool.181, 281–286 (1972)Google Scholar
  16. Singer, M.: The influence of the nerve in regeneration of the amphibian extremity. Quart. Rev. Biol.27, 169–200 (1952)Google Scholar
  17. Singer, M., Craven, L.: The growth and morphogenesis of the regenerating forelimb of the adultTriturus following denervation at various stages of development. J. exp. Zool.108, 279–308 (1948)Google Scholar
  18. Singer, M., Salpeter, M. M.: Regeneration in vertebrates: The role of the wound epithelium. In: Growth in living systems (Zarrow, M., ed.). New York: Basic Books 1961Google Scholar
  19. Schmidt, A. J.: Forelimb regeneration of thyroidectomized adult newts. I. Morphology. J. exp. Zool.137, 197–226 (1958a)Google Scholar
  20. Schmidt, A. J.: Forelimb regeneration of thyroidectomized adult newts. II. Histology. J. exp. Zool.139, 95–136 (1958b)Google Scholar
  21. Schmidt, A. J.: Molecular basis of regeneration: Enzymes. Illinois Monographs in Medical Sciences, vol. 6, No. 4. Urbana: Illinois Press 1966Google Scholar
  22. Schmidt, A. J.: Cellular biology of vertebrate regeneration and repair. Chicago: Chicago Univ. Press 1968Google Scholar
  23. Schotte, O. E., Butler, E. G.: Phases in regeneration of the urodele limb and their dependence upon the nervous system. J. exp. Zool.97, 95–121 (1944)Google Scholar
  24. Schotte, O. E., Hall, A. B.: Effect of hypophysectomy upon phases of regeneration in progress (Triturus viridescens). J. exp. Zool.121, 521–560 (1952)Google Scholar
  25. Spallanzani, A.: An essay on animal reproductions, 1768. (Translated from the Italian by M. Maty, London, 1769)Google Scholar
  26. Tassava, R. A., Goss, R. J.: Regeneration rate and amputation level in fish fins and lizard tails. Growth30, 9–21 (1966)Google Scholar
  27. Wolpert, L.: Positional information and pattern formation. In: Current topics in developmental biology, vol. 6 (Moscona, A. A., and Monroy, A., eds.), p. 183–224. New York: Academic Press 1971Google Scholar
  28. Zeleny, C.: Studies on the factors controlling the rate of regeneration. Illinois Biological Monographs, vol. 3, No. 1. Urbana: University of Illinois Press 1916Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Laurie E. Iten
    • 1
  • Susan V. Bryant
    • 1
  1. 1.Department of Developmental and Cell Biology and Center for PathohiologyUniversity of CaliforniaIrvine, Irvine

Personalised recommendations