Chemistry of Natural Compounds

, Volume 19, Issue 6, pp 727–729 | Cite as

DNA-cellulose — A new affinity sorbent for human leucocytic interferon

  • G. T. Mavlanov
  • S. A. Auelbekov
  • D. B. Dzhumaev
  • Kh. A. Aslanov
Article
  • 32 Downloads

Abstract

The possibility has been studied of using affinity chromatography on DNA-cellulose for the purification of interferon. It has been shown that interferon at a low ionic strength binds to DNA-cellulose, and on this basis a new method is proposed for purifying interferon which permits its 200-fold purification with small losses.

Keywords

Interferon Affinity Chromatography Newcastle Disease KSCN Linear NaCI Gradient 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    A. S. Sadykov, F. I. Ershov, et al., Interferon Inductors [in Russian], Tashkent (1978).Google Scholar
  2. 2.
    F. I. Ershov and A. S. Novokhatskii, Interferon and Its Inductors [in Russian], Moscow (1981).Google Scholar
  3. 3.
    V. D. Solov'ev and T. A. Bektemirov, Interferon in the Theory and Practice of Medicine [in Russian], Moscow (1970).Google Scholar
  4. 4.
    K. Cantell and S. Hirvonen, J. Gen. Virol.,39, 541 (1978).CrossRefGoogle Scholar
  5. 5.
    K. Berg and I. Heron, Scand. J. Immunol.,11, 489 (1980).CrossRefGoogle Scholar
  6. 6.
    M. Rubenstein et al., Science,202, 1289 (1978).CrossRefGoogle Scholar
  7. 7.
    K. C. Zoon, M. E. Smith, et al., Proc. Natl. Acad. Sci. USA,76, No. 11, 5601 (1979).CrossRefGoogle Scholar
  8. 8.
    R. G. Parsons, P. H. Aldenderfer, and R. Kowal, J. Natl. Cancer Inst.,63, No. 1, 43 (1979).PubMedGoogle Scholar
  9. 9.
    R. M. Litman et al., J. Biol. Chem., No. 243, 6222 (1968).Google Scholar
  10. 10.
    O. H. Lowry et al., J. Biol. Chem.,193, 265 (1957).Google Scholar

Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • G. T. Mavlanov
  • S. A. Auelbekov
  • D. B. Dzhumaev
  • Kh. A. Aslanov

There are no affiliations available

Personalised recommendations