The possibility has been studied of using affinity chromatography on DNA-cellulose for the purification of interferon. It has been shown that interferon at a low ionic strength binds to DNA-cellulose, and on this basis a new method is proposed for purifying interferon which permits its 200-fold purification with small losses.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
A. S. Sadykov, F. I. Ershov, et al., Interferon Inductors [in Russian], Tashkent (1978).
F. I. Ershov and A. S. Novokhatskii, Interferon and Its Inductors [in Russian], Moscow (1981).
V. D. Solov'ev and T. A. Bektemirov, Interferon in the Theory and Practice of Medicine [in Russian], Moscow (1970).
K. Cantell and S. Hirvonen, J. Gen. Virol.,39, 541 (1978).
K. Berg and I. Heron, Scand. J. Immunol.,11, 489 (1980).
M. Rubenstein et al., Science,202, 1289 (1978).
K. C. Zoon, M. E. Smith, et al., Proc. Natl. Acad. Sci. USA,76, No. 11, 5601 (1979).
R. G. Parsons, P. H. Aldenderfer, and R. Kowal, J. Natl. Cancer Inst.,63, No. 1, 43 (1979).
R. M. Litman et al., J. Biol. Chem., No. 243, 6222 (1968).
O. H. Lowry et al., J. Biol. Chem.,193, 265 (1957).
Institute of Bioorganic Chemistry, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 766–768, November–December, 1983.
About this article
Cite this article
Mavlanov, G.T., Auelbekov, S.A., Dzhumaev, D.B. et al. DNA-cellulose — A new affinity sorbent for human leucocytic interferon. Chem Nat Compd 19, 727–729 (1983). https://doi.org/10.1007/BF00575180
- Affinity Chromatography
- Newcastle Disease
- Linear NaCI Gradient