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Preparation of homogeneous protamines by the fractionation of nucleoprotamine

Abstract

A new method has been developed for obtaining individual protamines which is based on the chromatographic separation of an ultrasonically treated nucleoprotamine solution. The separation of the nucleoprotamine from the gonads of the Caspian sturgeonAcipenser stellatus on CM-Sephadex G-25 led to the isolation of three fractions. Analysis showed that two fractions contained homogeneous protamine — stellins A and B. The third fraction contained nonprotamines and DNA.

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Additional information

Moscow Technological Institute of the Meat and Dairy Industry. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 378–381, May–June, 1985.

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Makarov, N.V., Yaglitskaya, N.N. & Rybin, V.K. Preparation of homogeneous protamines by the fractionation of nucleoprotamine. Chem Nat Compd 21, 353–355 (1985). https://doi.org/10.1007/BF00574211

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Keywords

  • Organic Chemistry
  • Chromatographic Separation
  • Protamine