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A cytological and quantitative study of neoblasts in the naidOphidonais serpentina (Oligochaeta)

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The fine structure of the neoblasts in the naidOphidonais serpentina has been examined. The neoblasts of control worms have a relatively large nucleus, containing a large nucleolus, a sparse amount of rough endoplasmic reticulum, and an abundance of free ribosomes and mitochondria. Although Golgi membranes have been demonstrated, there is no evidence that the neoblasts are secretory in nature. Neoblasts form loose cell-to-cell contacts with one another and with peritoneal cells. In worms 12 hours after posterior transection, the neoblasts found at the end of the severed ventral nerve cord have rounded up and are no longer spindle-shaped. Counts of neoblasts immediately after posterior transection indicate that they are equally distributed in the last five segments. A statistical analysis of their distribution during posterior regeneration reveals a significant increase in neoblasts in the last three segments and a migration of neoblasts toward the wound. The role of neoblasts in oligochaete posterior regeneration is discussed.

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  1. Abel, M.: Beiträge zur Kenntnis der Regenerationsvorgänge bei den limnicolen Oligochäten. Z. wiss. Zool.73, 1–76 (1902)

  2. Boilly, B.: Êtude ultrastructurale de l'evolution des tissue impliqués dans la régénération céphalique et caudale deSyllis amica Q. (annélide polychète). 1. La dédifferenciation. 2. L'activation et la différenciation. J. Microscopie7, 865–894 (1968a)

  3. Boilly, B.: Origin des cellules de régénération chezAricia foetida Clap. (annélide polychète). Arch. Anat. micr. Morph. exp.57, 297–308 (1968b)

  4. Boilly, B.: Sur l'origine des cellules régénératrices chez les Annélides Polychaètes. Arch. Zool. exp. gen.110, 125–144 (1969)

  5. Chapron, C.: Êtude histologique, infrastructurale et expérimentale de la régénération céphalique chez le lombricienEisenia foetida unicolor (Sav.). Ann. Emb. Morph.3, 235–250 (1970)

  6. Foulkes, R. H.: Regeneration of the anterior end ofAulophorus furcatus (Naididae) with special reference to the effect of x-rays. Biol. Bull.105, 80–86 (1953)

  7. Hämmerling, J.: Die ungeschlechtliche Fortpflanzung und Regeneration beiAeolosoma hemprichii. Zool. Jb. Abt. allg. Zool. u. Physiol.41, 591–656 (1924)

  8. Harvey, W. R.: Least squares analysis of data. Agricultural Res. Service U. S. D. A. AR-20-8 (1960)

  9. Hepke, P.: Über histo- und organogenetische Vorgänge bei den Regenerationsprozessen der Naiden. Z. wiss. Zool.63, 263–291 (1897)

  10. Herlant-Meewis, H.: Contribution à l'étude de la régénération chez les Oligochètes. Reconstitution du germen chezLumbricillus lineatus (Enchytraeides). Arch. Biol. (Liège)57, 197–306 (1946)

  11. Hill, S. D.: Origin and development of the regeneration blastema in the fanwormSabella melanostigma. Amer. Zool.9, 595–096 (1969a)

  12. Hill, S. D.: Origin and development of the regeneration blastema in some species of sedentary polychaetes. Ph. D. Thesis, Univ. of Michigan 1969b

  13. Hill, S. D.: Origin of the regeneration blastema in polychaete annelids. Amer. Zool.10, 101–112 (1970)

  14. Iwanow, P.: Die Regeneration von Rumpf- und Knopfsegmenten beiLumbriculus variegatus Gr. Z. wiss. Zool.75, 327–390 (1903)

  15. Krecker, F. H.: Some phenomena of regeneration inLimnodrilus and related forms. Z. wiss. Zool.95, 383–448 (1910)

  16. Krecker, F. H.: Origin and activities of the neoblasts in the regeneration of microdrilous Annelida. J. exp. Zool.37, 27–46 (1923)

  17. Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409–414 (1961)

  18. Michel, A.: Recherches sur les régénérations chez les annélides. Bull. Sci. Fr. Belg.31, 245–420 (1898)

  19. Moment, G. B.: Recovery and abscopal effects after inhibitory x-irradiation in earthworm regeneration. J. exp. Zool.181, 33–40 (1972)

  20. Morita, M., Best, J. B., Noel, J.: Electron microscopic studies of planarian regeneration. I. Fine structure of neoblasts inDugesia dorotocephala. J. Ultrastruct. Res.27, 7–23 (1969)

  21. Nakagawa, S., Yamamoto, I.: A simple method for preparation of phosphate buffered formaldehyde fixative. J. Electron Microsc.19, 389–390 (1970)

  22. O'Brien, J. P.: Studies on the effects of x-rays on regeneration in the fragmenting oligochaeteNais paraguayensis. Growth6, 203–229 (1942)

  23. O'Brien, J. P.: Studies on the cellular basis of regeneration inNais paraguayensis, and the effects of x-ray thereon. Growth10, 25–44 (1946)

  24. Pederson, K. G.: Cytological studies on the planarian neoblast. Z. Zellforsch.50, 799–815 (1959)

  25. Pederson, K. G.: Studies on regeneration blastemas of the planarianDugesia tigrina with special reference to differentiation of the muscle-connective tissue filament system. Wilhelm Roux' Archiv169, 134–169 (1972)

  26. Pennak, R. W.: Fresh-water invertebrates. New York: Ronald Press 1953

  27. Potswald, H. E.: Cytological observations on the so-called neoblasts in the serpulidSpirorbis. J. Morph.128, 241–259 (1969)

  28. Potswald, H. E.: The relationship of early oocytes to putative neoblasts in the serpulidSpirorbis borealis. J. Morph.137, 215–228 (1972)

  29. Randolph, H.: The regeneration of the tail inLumbriculus. Zool. Anz.14, 154–156 (1891)

  30. Randolph, H.: The regeneration of the tail inLumbriculus. J. Morph.7, 317–345 (1892)

  31. Richardson, K. C., Jarett, L., Finke, E. H.: Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol.35, 313–323 (1960)

  32. Sayles, L. P.: Origin of the mesoderm and behavior of the nucleolus in regeneration inLumbriculus. Biol. Bull.52, 278–313 (1927)

  33. Steen, T. P.: Origin and differentiative capacities of cells in the blastema of the regenerating salamander limb. Amer. Zool.10, 119–132 (1970)

  34. Stéphan-Dubois, F.: Les néoblastes dans la régénération postérieure des oligochetes microdriles. Bull. Biol.88, 181–247 (1954)

  35. Stéphan-Dubois, F.: Migration et différenciation des néoblastes dans la régénération antérieure deLumbriculus variegatus (Annélide Oligochète). C. R. Soc. Biol. (Paris)150, 1239 (1956)

  36. Stone, R. G.: The effects of x-ray on regeneration inTubifex tubifex. J. Morph.53, 389–431 (1932)

  37. Stone, R. G.: The effects of x-ray on anterior regeneration inTubifex tubifex. J. Morph.54, 303–320 (1933)

  38. Turner, C. D.: The effects of x-rays on posterior regeneration inLumbriculus inconstant. J. exp. Zool.68, 95–119 (1934)

  39. Turner, C. D.: The effects of x-rays on anterior regeneration inLumbriculus inconstans. J. exp. Zool.71, 53–82 (1935)

  40. Venable, G. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol.25, 407–408 (1965)

  41. Wagner, F.: Beiträge zur Kenntnis der Regenerationsprozesse beiLumbriculus variegatus. II. Teil. Zool. Jh. Abt. Anat. u. Ontog.136, 255–318 (1906)

  42. Ward, H. B., Whipple, G. C.: Fresh water biology. New York: John Wiley and Sons Inc. 1918

  43. Wissocq, J. C.: Êtude ultrastructurale de l'évolution des muscles longitudinaux lors de la stolonisation expérimentale deSyllis amica (Quatrefages) (Annélide Polychète). Z. Zellforsch.83, 449–467 (1967)

  44. Zhinkin, L.: Über die Bedeutung der Mesodermanlagen bei der Regeneration vonRhynchelnis limosella (Untersuchungen über die Wirkung der Röntgenstrahlen auf Regeneration und Transplantation). Zool. Anz.105, 305–312 (1934)

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Work supported by a grant (GB-18429) from N. S. F.

The authors wish to thank Dr. Richard A. Damon for his help and guidance in making the statistical analysis and Dr. Joseph G. Kunkel for reviewing the manuscript and for offering helpful advice.

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Bilello, A.A., Potswald, H.E. A cytological and quantitative study of neoblasts in the naidOphidonais serpentina (Oligochaeta). W. Roux' Archiv f. Entwicklungsmechanik 174, 234–249 (1974). https://doi.org/10.1007/BF00573227

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  • Endoplasmic Reticulum
  • Developmental Biology
  • Fine Structure
  • Quantitative Study
  • Rough Endoplasmic Reticulum