Virus Genes

, Volume 12, Issue 2, pp 149–154 | Cite as

Molecular cloning and sequence determination of the complete genome of the virulent echovirus 9 strain Barty

  • Holger Zimmermann
  • Hans J. Eggers
  • Birgit Nelsen-Salz


As part of a study of the molecular basis of pathogenicity of echovirus 9, the complete nucleotide sequence of the mouse-virulent echovirus 9 strain Barty was determined. Excluding the poly(A) tail, the complete RNA genome is composed of 7451 bases. The postulated open reading frame extends from nucleotide (nt) 741 to 7349 and predicts a polyprotein of 2203 amino acids (aa). As compared with the sequence of the echovirus 9 prototype strain Hill, which is apathogenic for newborn mice, 1492 nt are exchanged, leading to 9% divergence of the deduced amino acid sequence. The foremost difference between both strains is located at the C-terminus of the capsid protein VP1. In the case of strain Barty, an additional 10 aa fragment, including an RGD motif, is inserted.

Key words

echovirus 9 genomic sequence RGD-motif pathogenicity 


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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Holger Zimmermann
    • 1
  • Hans J. Eggers
    • 1
  • Birgit Nelsen-Salz
    • 1
  1. 1.Institut für Virologie der Universität zu KölnCologneGermany

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