Cloning of theHIS3 gene ofYarrowia lipolytica
Article
Received:
Accepted:
- 41 Downloads
- 1 Citations
Abstract
TheHIS3 gene of the yeastYarrowia lipolytica has been cloned from a genomic library by complementation of thehis3 mutation ofSaccharomyces cerevisiae. The gene was subsequently subcloned inEscherichia coli and characterized by restriction enzyme mapping.
Key words
HIS3 Yarrowia lipolytica yeastPreview
Unable to display preview. Download preview PDF.
References
- Abdelal ATH, Kennedy EH & Ahearn DG (1977) Purification and characterization of a neutral protease fromSaccharomycopsis lipolytica. J. Bacteriol. 130 (3): 1125–1129Google Scholar
- Beggs JD (1978) Transformation of yeast by a replicating hybrid plasmid. Nature, Lond. 275: 104–109Google Scholar
- Birnboim HC & Doly J (1979) A rapid alkaline extraction for screening recombinant plasmid DNA. Nucleic Acid Research 7 (6): 1513–1523Google Scholar
- Cheng SC & Ogrydziak DM (1986) Extracellular RNase produced byYarrowia lipolytica. J. Bacteriol. 168: 581–589.Google Scholar
- Cohen SN, Chang ACY, Boyer HW & Helling RB (1973) Construction of biologically functional bacterial plasmids in vitro. Proc. Natn. Acad. Sci. USA 70: 3240–3244Google Scholar
- Cryer R, Eccleshall R & Marmur J (eds) (1975) Isolation of yeast DNA. In: Methods of Cell Biology, Preparation of Yeast Spheroplasted Strains, Vol 12 (pp 39–44). Academic Press, New YorkGoogle Scholar
- Davidow LS, Apostolakos D, O'Donnell MM, Proctor AR, Ogrydziak DM, Wing RA, Stasko I & DeZeeuw JR (1985) Integrative transformation of the yeastYarrowia lipolytica. Curr. Genet. 10: 3948Google Scholar
- Davidow LS, Kaczmarek FS, DeZeeuw JR, Conlon SW, Lauth MR, Pereira DA & Franke AE (1987) TheYarrowia LEU2 gene. Curr. Genet. 11: 377–383Google Scholar
- Hinnen A, Hicks J & Fink G (1978) Transformation of yeast. Proc. Natn. Acad. Sci. USA 75: 1929–1933Google Scholar
- Ito H, Fukada Y, Murata K & Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J. Bacteriol 15: 163–168Google Scholar
- Gaillardin CM & Ribet AM (1987)LEU2 Directed expression of B-galactosidase activity and phleomycin resistance inYarrowia lipolytica. Curr. Genet. 11: 369–375Google Scholar
- Gaillardin CM, Riset AM & Heslot H (1985) Integrative transformation of the yeastYarrowia lipolytica. Curr. Genet. 10: 39–48Google Scholar
- Maniatis T, Jeffrey A & Kleid DG (1975) Nucleotide sequence of the rightward operator of phage Lambda. Proc. Natl. Acad. Sci. USA 72: 1184–1188Google Scholar
- Maniatis T, Fritsch EF & Sambrook J (Eds) (1982) Molecular Cloning, a Laboratory Manual. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
- Ogrydziak DM & Scharf SJ (1982) Alkaline extracellular protease produced bysaccharomycopsis lipolytica CX 161-1B. J. Gen. Microbiol. 128: 1225–1234Google Scholar
- Ogrydziak DM, Suk-Chung Cheng & Scharf SJ (1982) Characterization ofSaccharomycopsis lipolytica mutants producing lowered levels of alkaline extracellular protease. J. Gen. Microbiol. 128: 2271–2280Google Scholar
- Prodromou C (1988) Genetical and biochemical studies on extracellular proteases ofYarrowia lipolytica and other yeast. PhD thesisGoogle Scholar
- Rigby PWJ, Dieckmann M, Rhodes C & Berg P (1977) Labelling deoxyribonucleic acid to high specificity. J. Mol. Biol. 113: 237–251Google Scholar
- Sherman F, Fink GR & Hicks JB (Eds) (1986) Methods in Yeast Genetics. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
- Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517Google Scholar
- Struhl K & Davis RW (1980) A physical, genetic and transcriptional map of the clonedHIS3 gene region ofSaccharomyces cerevisiae. J. Mol. Biol. 130: 309–332Google Scholar
- Struhl K, Cameron JR & Davis RW (1976) Functional genetic expression of eukaryotic DNA inEscherichia coli. Proc. Natn. Acad. Sci. USA 73: 1471–1475Google Scholar
- Yamada T & Ogrydziak DM (1983) Extracellular acid proteases produced bySaccharomycopsis lipolytica. J. Bacteriol. 154: 23–31Google Scholar
Copyright information
© Kluwer Academic Publishers 1991