Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Quantitative-morphometric aspects of bergmann glial (Golgi epithelial) cell development in rats

A golgi study

  • 75 Accesses

  • 17 Citations

Summary

Bergmann glial (Golgi epithelial) cells in the cerebella of rats of various ages were stained by the rapid Golgi technique, and their radial stem processes were measured for length and diameter. Additionally, the average number of such processes per cell was counted, and the development of bushy lateral protrusions was quantified. The length of radial processes—depending on the thickness of the molecular layer—was found to increase up to the end of the 2nd year of life. This elongation was accompanied by a reduction of the mean process diameter which was, however, not sufficient to prevent an increase in the cytoplasmic volume of the elongating cells. A marked outgrowth of lateral protrusions was observed up to at least the 5th month of life. These data are compared with earlier findings on the development of rat brain stem fetal radial glia, and of rabbit retinal Müller cells. Common mechanisms of glial cell development are discussed.

This is a preview of subscription content, log in to check access.

References

  1. Björklund H, Eriksdotter-Nilsson M, Dahl D, Rose G, Hoffer B, Olson L (1985) Image analysis of GFA-positive astrocytes from adolescence to senescence. Exp Brain Res 58:163–170

  2. Bruni JE, Clattenburg RE, Millar E (1983) Tanycyte ependymal cells in the third ventricle of young and adult rats: a Golgi study. Anat Anz 153:53–68

  3. Chan-Palay V, Palay SL (1972) The form of velate astrocytes in the cerebellar cortex of monkey and rat: high-voltage electron microscopy of rapid Golgi preparations. Z Anat Ent Gesch 138:1–19

  4. Das GD (1976) Differentiation of Bergmann glia cells in the cerebellum: a Golgi study. Brain Res 110:199–213

  5. DelCerro M, Swarz JR (1976) Prenatal development of Bergmann glial fibres in rodent cerebellum. J Neurocytol 5:669–676

  6. Eberhardt W, Reichenbach A (1986) Spatial buffering of potassium by retinal Müller (glial) cells of various morphology calculated by a model. Neuroscience, in press

  7. Fülöp Z, Lakos J, Bascó E, Hajós F (1979) Identification of early glial elements as the precursors of Bergmann glia. A Golgi analysis of the developing rat cerebellar cortex. Acta Morphol Acad Sci Hung 27:273–280

  8. Gardner-Medwin AR (1984) A foot in the vitreous fluid. Nature [Lond] 309:113

  9. Hajós F, Bascó E (1984) Surface-contact glia. Springer-Verlag, Berlin Heidelberg New York, pp 54–57

  10. Harris RM (1985) Light microscopic depth measurements of thick sections. J Neurosci Methods 14:97–100

  11. Haug H, Kölln M, Rast A (1976) The postnatal development of myelinated nerve fibres in the visual cortex of the rat. Cell Tissue Res 167:265–288

  12. Ito M (1984) The cerebellum and neural control. Raven Press, New York

  13. Landfield PW, Rose G, Sandles L, Wohlstadter TC, Lynch G (1977) Patterns of astroglial hypertrophy and neuronal degeneration in the hippocampus of aged, memory-deficient rat. J Gerontol 32:3–12

  14. Lange W (1976) The myelinated parallel fibres of the cerebellar cortex and their regional distribution. Cell Tissue Res 166:489–496

  15. Leibnitz L (1967) Die Veränderung von Gewicht, Volumen und spezifischem Gewicht des Rattengehirns nach Fixierung, Dehydrierung und Aufhellung. J Hirnforsch 9:97–104

  16. Lewis PD, Fülöp Z, Hajós F, Balazs R, Woodhams PL (1977) Neuroglia in the internal granular layer of the developing rat cerebellar cortex. Neuropathol Appl Neurobiol 3:183–190

  17. Lipton P, Heimbach CJ (1978) Mechanism of extracellular potassium stimulation of protein synthesis in the in vitro hippocampus. J Neurochem 31:1299–1307

  18. Mugnaini E (1972) The histology and cytology of the cerebellar cortex. In: Larsell O, Jansen J (eds) The Comparative Anatomy and Histology of the Cerebellum. University of Minnesota Press, Minneapolis

  19. Newman EA (1984) Regional specialization of retinal glial cell membrane. Nature 309:155–157

  20. Newman EA (1985) Membrane physiology of retinal glial (Müller) cells. J Neurosci 5:2225–2239

  21. Newman EA (1986a) Potassium conductance hot-spots in mammalian Müller (glial) cells. Soc Neurosci [Abstr] 12:633

  22. Newman EA (1986b) High potassium conductance in astrocyte endfeet. Science 233:453–454

  23. Newman EA, Frambach DA, Odette LL (1984) Control of extracellular potassium levels by retinal glial cell K+ siphoning. Science 225:1174–1175

  24. Nilius B, Reichenbach A (1987) Efficient K+ buffering by mammalian retinal glial cells is due to cooperation of specialized ion channels (submitted)

  25. Pellionisz A, Llinás R (1977) A computer model of cerebellar Purkinje cells. Neuroscience 2:37–48

  26. Pouwels E (1978) On the development of the cerebellum of the trout, Salmo gairdeneri. V. Neuroglial cell development. Anat Embryol 3:67–83

  27. Rall W (1977) Core conductor theory and cable properties of neurons. In: Brookhart JM, Mountcastle VB (eds) Handbook of Physiology, Sect 1: The Nervous System, vol 1: Cellular Biology of Neurons, Part 1. Am Physiol Soc, Bethesda

  28. Ramon y Cajal S (1955) Histologie du Système Nerveux de l'Homme et des Vertébrés. CSIC, Madrid (Reprint of 1909–1911 Edition)

  29. Reichenbach A, Dettmer D, Reichelt W, Eberhardt W (1985) Na+, K+-activated adenosine triphosphatase of isolated Müller cells from the rabbit retina shows a K+ dependence similar to that of brain astrocytes. Neurosci Lett 59:281–284

  30. Reichenbach A, Neumann M, Brückner G (1987) Cell length to diameter relation of rat fetal radial glia—does impaired K+ transport capacity of long thin cells cause their perinatal transformation into multipolar astrocytes? Neurosci Lett 73:95–100

  31. Reichenbach A, Reichelt W (1986) Postnatal development of radial glial (Müller) cells of the rabbit retina. Neurosci Lett 71:125–130

  32. Reichenbach A, Wohlrab F (1986) Morphometric parameters of Müller (glial) cells dependent on their topographic localization in the nonmyelinated part of the rabbit retina. A consideration on functional aspects of radial glia. J Neurocytol 15:451–459

  33. Seress L, Bascó E, Hajós F, Fülöp Z (1978) The effect of thyroid hormone on the formation of rat cerebellar Bergmann-glia. Acta Morphol Acad Sci Hung 26:95–100

  34. Shiga T, Ichikawa M, Hirafa Y (1983a) A Golgi study of Bergmann glial cells in developing rat cerebellum. Anat Embryol 167:191–201

  35. Shiga T, Ichikawa M, Hirata Y (1983b) Spatial and temporal pattern of postnatal proliferation of Bergmann glial cells in rat cerebellum: an autoradiographic study. Anat Embryol 167:203–211

  36. Ugrumov MV, Chandrasekhar K, Borisova NA, Mitskevich MS (1979) Light and electron microscopical investigations on the tanycyte differentiation during the postnatal period in the rat. Cell Tissue Res 201:295–303

Download references

Author information

Correspondence to Andreas Reichenbach.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hanke, S., Reichenbach, A. Quantitative-morphometric aspects of bergmann glial (Golgi epithelial) cell development in rats. Anat Embryol 177, 183–188 (1987). https://doi.org/10.1007/BF00572543

Download citation

Key words

  • Glia
  • Cerebellum
  • Rat
  • Development
  • Morphometry