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Degeneration of the gonococcus (in various media)

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Summary

The ultrastructure of the gonococcus cultivated in different synthetic media and inoculated into the peritoneal cavity of the mouse makes it possible to distinguish the various degenerative morphological states of the microbe.

A comparative study of phagocytosis and transformation of the gonococcus by leukocytes together with the changes observed in artificial media discloses a certain resemblance between the different stages of the progression towards L forms.

Reversion to normal forms is only possible if gonococcal degeneration has not reached an extreme point and if the new medium compensates for the unfavourable conditions which produced L transformation.

Zusammenfassung

Die Ultrastruktur von Gonokokken, die in verschiedenen synthetischen Medien kultiviert und dann in dem Peritonialraum der Maus inokkuliert wurden, gibt die Möglichkeit die verschiedenen Degenerationsstadien der Gonokokken vom Strukturellen her zu beobachten. Eine vergleichende Studie der Phagocytose und Transformation der Gonokokken durch Leukocyten zusammen mit Veränderungen, die im Kulturmedium beobachtet wurden lassen eine gewisse Ähnlichkeit in der Entwicklung zu den L-Formen erkennen. Eine Reversibilität solcher Veränderungen ist nur dann zu erreichen wenn solche degenerativen Veränderungen nicht extrem vorhanden sind, und zusätzlich das Kulturmedium, in welches diese degenerierten Formen gegeben werden ein Kompensierungsmedium für die Faktoren darstellt, welche die L-Transformation verursacht haben.

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References

  1. 1.

    Bacigalupi, B. A., Lawson, J. W.: Defined physiolological conditions for the induction of the L-form of Neisseria gonorrhoeae. J. Bact.116, 778–784 (1973)

  2. 2.

    Dienes, L., Bandur, B. M., Madoff, S.: Development of L-type growth in Neisseria gonorrhoeae cultures. J. Bact.87, 1471–1476 (1964)

  3. 3.

    Gnarpe, H., Wallin, J., Forsgren, A.: Studies in venereal disease. I. Isolation of L-phase organisms of N. gonorrhoeae from patients with gonorrhoea. Brit. J. Vener. Dis.48, 496–499 (1972)

  4. 4.

    Jephcott, A. E., Reyn, A.: Neisseria gonorrhoeae. Colony variation I. Acta path. microbiol. scand., Sect B79, 609–614 (1971)

  5. 5.

    Kellogg, D. S., Peacock, W. L., Deacon, W. E., Brown, L., Pirkle, C. I.: I. Virulence genetically linked to clonal variation. J. Bacteriol.85, 1274–1279 (1963)

  6. 6.

    Martin, R. R., Crowder, J. G., White, A.: Human reactions to staphylococcal antigens. A possible role of leukocyte lysosomal enzymes. J. Immunol.99, 269–275 (1967)

  7. 7.

    Martin, J. E., Lester, A. P.: Transgrow, a medium for transport and growth of Neisseria gonorrheae and Neisseria meningitidis. H. S. M. H. A. Hlth Rep.86, 30–33 (1971)

  8. 8.

    Morse, S. A., Bartenstein, L.: Factors affecting autolysis of Neisseria gonorrhoeae. Proc. Soc. Exp. Biol. Med.145, 1418–1421 (1974)

  9. 9.

    Novotny, P., Short, J. A., Walker, P. D.: An electron-microscope study of naturally occurring and cultured cells of Neisseria gonorrhoeae. J. Med. Microbiol.8, 413–427 (1974)

  10. 10.

    Ovčinnikov, N. M., Delektorskij, F. V.: Electron microscope studies of gonococci in the urethral secretions of patients with gonorrhoea. Brit. J. Vener. Dis.47, 419–439 (1971)

  11. 11.

    Pariser, H., Farmer, A. D.: Diagnosis of gonorrhea in the asymptomatic female: Comparison of slide and culture technics. South. Med. J.61, 505–506 (1968)

  12. 12.

    Reynolds, E. S.: The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. cell. Biol.17, 208–212 (1963)

  13. 13.

    Sparling, P. F., Yobs, A. R.: Colonial morphology of Neisseria gonorrhoeae isolated from males and females. J. Bact.93, 513 (1967)

  14. 14.

    Swanson, J., Zeligs, B.: Studies on gonococcus infection. VI. Electron microscopic study on in vitro phagocytosis of gonococci by human leukocytes. Infect. and Immun.10, 645–656 (1974)

  15. 15.

    Swanson, J., King, G., Zeligs, B.: Studies on gonococcus infection. VII. In vitro killing of gonococci by human leukocytes. Infect. and Immun.11, 65–68 (1975)

  16. 16.

    Thayer, J. D., Martin, J. E.: Improved medium selective for cultivation of gonorrhoeae and N. meningitidis. Publ. Hlth Rep. (Wash.)81, 559–562 (1966)

  17. 17.

    Tew, J. G., Hess, W. M., Donaldson, D. M.: Lysozyme and-lysin release stimulated by antigenantibody complexes and bacteria. J. Immunol.102, 743–750 (1969)

  18. 18.

    Waitkins, S. A., Flynn, J.: Intracellular growth and type variation of Neisseria gonorrhoeae in tissue cell-cultures. J. Med. Microbiol.6, 399–403 (1973)

  19. 19.

    Ward, M. E., Glynn, A. A., Watt, J. P.: The fate of gonococci in polymorphonuclear leucocytes: an electronmicroscope study of the natural disease. Brit. J. exp. Path.53, 289–294 (1972)

  20. 20.

    Wolf-Watz, H., Elmros, T., Normark, S., Bloom, G. D.: Cell envelope of Neisseria gonorrhoeae. A comparative study with Escherichia coli. Brit. J. vener. Dis.52, 142–145 (1976)

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We acknowledge with gratitude the expert technical assistance of Mr. Pierre Carraux

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Olmos, L. Degeneration of the gonococcus (in various media). Arch. Derm. Res. 256, 305–317 (1976). https://doi.org/10.1007/BF00572497

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Keywords

  • Public Health
  • Microbe
  • Normal Form
  • Peritoneal Cavity
  • Extreme Point