Acta Neuropathologica

, Volume 89, Issue 6, pp 471–474 | Cite as

Cu/Zn superoxide dismutase-like immunoreactivity is present in Lewy bodies from Parkinson disease: a light and electron microscopic immunocytochemical study

  • K. Nishiyama
  • S. Murayama
  • J. Shimizu
  • Y. Ohya
  • S. Kwak
  • K. Asayama
  • I. Kanazawa
Express Communication

Abstract

Copper-zinc superoxide dismutase (SOD1)-like immunoreactivity has been demonstrated in Lewy body-like inclusions (LIs) in brain tissues from patients with familial and sporadic amyotrophic lateral sclerosis. Using immunocytochemistry, we studied Lewy bodies (LBs), the original inclusions from which the term LI was derived, in five patients with Parkinson disease (PD). Surprisingly, many LBs were immunostained by an antibody against SOD1. There were two types of staining pattern: a diffuse pattern, and a peripheral pattern with an unstained core. An immunoelectron microscopic study demonstrated that the immunoreactive products were restricted to the fibrillary profiles, sparing the unstructured core. Our results showed that SOD1-like immunoreactivity occurred frequently in LBs and LIs, suggesting that a common cytopathological process is responsible for the formation of LB-type neuronal intracytoplasmic inclusions. Our results also suggest that SOD1 plays a role in the neurodegeneration associated with PD.

Key words

Superoxide dismutase Lewy body Parkinson disease Lewy body-like hyaline inclusion Amyotrophic lateral sclerosis 

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References

  1. 1.
    Asayama K, Burr IM (1984) Joint purification of mangano and cuprozinc superoxide dismutases from a single source: a simplified method. Anal Biochem 136:336–339Google Scholar
  2. 2.
    Asayama K, Janco RL, Burr IM (1985) Selective induction of manganous superoxide dismutase in human monocytes. Am J Physiol 249:393–397Google Scholar
  3. 3.
    Gurney ME, Pu H, Chiu AY, Dal Canto MC, Polchow CY, Alexander DD, Caliendo J, Hentati A, Kwon YW, Deng H, Chen W, Zhai P, Sufit RL, Siddique T (1994) Motor neuron degeneration in mice that express a human Cu, Zn superoxide dismutase mutation. Science 264:1772–1775Google Scholar
  4. 4.
    Kuzuhara S, Mori H, Izumiyama N, Yoshimura M, Ihara Y (1988) Lewy bodies are ubiquitinated. A light and electron microscopic immunocytochemical study. Acta Neuropathol (Berl) 75:345–353Google Scholar
  5. 5.
    Mori H, Kondo J, Ihara Y (1987) Ubiquitin is a component of paired helical filaments in Alzheimer's disease. Science 235: 1641–1644Google Scholar
  6. 6.
    Murayama S, Ookawa Y, Mori H, Nakano I, Ihara Y, Kuzuhara S, Tomonaga M (1989) Immunocytochemical and ultrastructural study of Lewy body-like hyaline inclusions in familial amyotrophic lateral sclerosis. Acta Neuropathol 78: 143–152Google Scholar
  7. 7.
    Murayama S, Mori H, Ihara Y, Bouldin TW, Suzuki K, Tomonaga M (1990) Immunocytochemical and ultrastructural studies of lower motor neurons in amyotrophic lateral sclerosis. Ann Neurol 27:137–148Google Scholar
  8. 8.
    Price DL (1994) Models of motor neuron disease — etiologies, mechanisms and therapies (abstract). Brain Pathol 4:302Google Scholar
  9. 9.
    Rosen DR, Siddique T, Patterson D, et al (1993) Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis. Nature 362:59–62Google Scholar
  10. 10.
    Shibata N, Kobayashi M (1991) Immunohistochemical localization of superoxide dismutases in the central nervous system with various types of disorders. Neuropathology (Kyoto) 11: 247–248Google Scholar
  11. 11.
    Shibata N, Hirano M, Kobayashi K (1993) Immunohistochemical demonstration of Cu/Zn superoxide dismutase in the spinal cord of patients with familial amyotrophic lateral sclerosis. Acta Histochem Cytochem 26:619–621Google Scholar
  12. 12.
    Shibata N, Hirano A, Kobayashi M, Sasaki S, Kato T, Matsumoto S, Shiozawa Z, Komori T, Ikemoto A, Umahara T, Asayama K (1994) Cu/Zn superoxide dismutase-like immunoreactivity in Lewy body-like inclusions of sporadic amyotrophic lateral sclerosis. Neurosci Lett 179:149–152Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • K. Nishiyama
    • 1
  • S. Murayama
    • 1
  • J. Shimizu
    • 1
  • Y. Ohya
    • 1
  • S. Kwak
    • 1
  • K. Asayama
    • 2
  • I. Kanazawa
    • 1
  1. 1.Department of Neurology, Institute for Brain Research, Faculty of MedicineUniversity of TokyoTokyoJapan
  2. 2.Department of PediatricsYamanashi Medical CollegeYamanashiJapan

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