Binding of colchicine to a soluble fraction of carrot cells grown in suspension culture
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Abstract
The binding of [3H]colchicine to soluble component prepared from carrot (Daucus carota L.) cells in suspension culture was assayed by the diethylaminoethyl(DEAE)-cellulose powder method. The binding activity was very labile and the time course of the binding indicated that the colchicine-bound complex was also unstable. The reaction was enhanced by vinblastine but lumicolchicine had no effect. The optimum temperature for the reaction was 30° C, and the colchicine binding constant was calculated to be 3.5·104 l mol−1 at 30° C.
Key words
Cell culture Colchicine Daucus TubulinPreview
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