Planta

, Volume 149, Issue 4, pp 350–354 | Cite as

Binding of colchicine to a soluble fraction of carrot cells grown in suspension culture

  • Shoji Okamura
Article

Abstract

The binding of [3H]colchicine to soluble component prepared from carrot (Daucus carota L.) cells in suspension culture was assayed by the diethylaminoethyl(DEAE)-cellulose powder method. The binding activity was very labile and the time course of the binding indicated that the colchicine-bound complex was also unstable. The reaction was enhanced by vinblastine but lumicolchicine had no effect. The optimum temperature for the reaction was 30° C, and the colchicine binding constant was calculated to be 3.5·104 l mol−1 at 30° C.

Key words

Cell culture Colchicine Daucus Tubulin 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Shoji Okamura
    • 1
  1. 1.Faculty of Pharmaceutical SciencesToyama Medical and Pharmaceutical UniversityToyamaJapan

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