Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Effect of sialic acid on glycation-induced fluorescence of albumin

  • 46 Accesses

Abstract

The aims of this study were to determine whether in vitro nonenzymatic glycation of proteins by sialic acid (sialylation) induces the generation of fluorescence, and whether the presence of this keto sugar may affect the generation of fluorescence induced by other sugars. Incubation of bovine serum albumin (BSA; 1.5 mM) with sugars (50 mM in 0.2 M phosphate buffer, pH 7.4, at 37°C) resulted in a time-dependent increase of formaldehyde release (moles/moles of protein). On the 17th day of incubation, the value was 0.53±0.06, 0.78±0.15 and 1.23±0.18 for sialic acid, fructose and glucose respectively, compared with 0.37±0.05 for BSA. The fluorescence intensity (arbitrary units/mg protein) was higher after 17 days of incubation with fructose (16.9±1.8) than with glucose (12.7±1.3), while no significant increase was noted with sialic acid compared with BSA (3.8±0.4). Fluorescence intensity increase by incubation with glucose (50 mM) was significantly reduced by sialic acid (20 mM) after both 10 (P<0.001) and 14 (P<0.001) days of incubation, while inhibition was weaker after 14 (P<0.05) than after 10 (P<0.001) days when fructose (50 mM) was used as the glycating agent. This indicates that sialic acid can be potentially used to limit the damage from adverse glycation-induced processes.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Brownlee M, Vlassara H, Cerami A, Non-enzymatic glycosylation and the pathogenesis of diabetic complications. Ann Intern Med 101:527–537, 1984

  2. 2.

    Suarez G, Nonenzymatic browning of proteins and the sorbitol pathway. In: Baynes JW, Monnier VM (eds) The Maillard reaction in aging, diabetes and nutrition. Liss, New York, pp 141–162, 1989

  3. 3.

    McKinney RA, Urbanowski JC, Dain JA, Nonenzymatic glycosylation of albumin and fetuin by sialic acid. Biochem Int 4:127–133, 1982

  4. 4.

    Oimoni M, Nakamichi T, Ohara T, Sakai M, Igaki N, Hata F, Baba S, Fructose-related glycation. Diabetes Res Clin Pract 7:137–139, 1989

  5. 5.

    Gallon PM, Fluckiger R, Hanneken A, Minishon MM, Gabbay KH, Chemical quantitation of glycosylation: fluorometric detection of formaldehyde release upon periodate oxidation of glycoglobin. Anal Biochem 117:427–432, 1981

  6. 6.

    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ, Protein measurement with Follin phenol reagent. J Biol Chem 193:265–275 1951

  7. 7.

    Suarez G, Rajaram R, Oronsky AL, Gawinowicz MA, Nonenzymatic glycation of bovine serum albumin by fructose (fructation). Comparison with the Maillard reaction initiated by glucose. J Biol Chem 164:3674–3679, 1989

  8. 8.

    McPherson JD, Shilton BH, Walton DJ, Role of fructose in glycation and cross-linking of proteins. Biochemistry 27: 1901–1907, 1988

  9. 9.

    Baynes JW, Role of oxidative stress in development of complications in diabetes. Diabetes 40:405–412, 1991

  10. 10.

    Bunn HF, Higgins PJ, Reaction of monosaccharides with proteins: possible evolutionary significance. Science 213:222–224, 1981

  11. 11.

    Suarez G, Maturana J, Oronsky AL, Raventos-Suarez C, Fructose-induced fluorescence generation of reductively methylated glycated bovine serum albumin: evidence for nonenzymatic glycation Amadori adducts. Biochim Biophys Acta 1075: 12–19, 1991

  12. 12.

    Ledeen RW, Yu RK, Chemistry and analysis of sialic acid. In: Rosenberg A, Schengrund CL (eds) Biological roles of sialic acid. Plenum, New York, pp 1–48, 1976

  13. 13.

    Kovacs IB, Born GVR, Inhibition of platelet thrombosis byN-acetyl neuraminic acid. Thromb Haemost 38:66–72, 1977

  14. 14.

    Patschke H, Selektive Aggregationshemmung — ein neues Konzept für Hemmstoffe der Thrombozytenfunktion. Klin Wochenschr 59:451–457, 1981

  15. 15.

    Shaklai N, Garlick RL, Bunn HF, Nonenzymatic glycosylation of human serum albumin alters its conformation and function. J Biol Chem 259:3812–3817, 1984

  16. 16.

    Wolff SP, Jiang ZY, Hunt JV, Protein glycation and oxidative stress in diabetes mellitus and aging. Free Radic Biol Med 10:339–352, 1991

Download references

Author information

Correspondence to V. Lipovac.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lipovac, V., Gavella, M. & Šverko, V. Effect of sialic acid on glycation-induced fluorescence of albumin. Acta Diabetol 31, 156–159 (1994). https://doi.org/10.1007/BF00570371

Download citation

Key words

  • Sialic acid
  • Glycation
  • Glucation
  • Fructation
  • Fluorescence