Wistar rats were injected intraperitoneally with 2-(14C)deoxyglucose and their thymuses were processed for thaw-mount autoradiography after 5, 10 or 35 min. Highest levels of radioactivity were demonstrated in the thymic medulla (5-fold higher than in the cortex). Scanning of autoradiograms for regional differences in grain densities indicated particularly intense glucose utilization in the cortico-medullary zone. Differences in glucose utilization between individual thymic zones seem to reflect differences in cellular composition, i. e., ratio of stroma cells to thymocytes.
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Brelińska R, Kaczmarek E, Warchol JB, Jaroszewski J (1985) Distribution of different cell types within the rat thymus in the neonatal period of life. Cell Tissue Res 240:473–478
Cunningham VJ, Cremer JE (1985) Current assumptions behind the use of PET scanning for measuring glucose utilization in brain. Trends Neurosci 8:96–99
Hwang WS, Ho TY, Luk SC, Simon GT (1974) Ultrastructure of the rat thymus. Lab Invest 31:473–487
Jemelin M, Frei J (1970) Leucocyte energy metabolism. III. Anaerobic and aerobic ATP production and related enzymes. Enzymol Biol Clin 11:289–323
Kaczmarek E (1986) Computer analysis of similarities and differences in cell morphology as related to lymphoid cells (doctor's thesis), Poznán
Pilgrim Ch, Wagner H-J (1981) Improving the resolution of 2-deoxy-d-glucose method. J Histochem Cytochem 29:190–194
Raviola E, Karnovsky MJ (1972) Evidence for a blood-thymus barrier using electron opaque tracers. J Exp Med 136:466–479
Rogers AW (1973) Techniques of autoradiography, 2nd edn. Elsevier, Amsterdam
Savino W, Dardenne M, Papiernik M, Bach J-F (1982) Thymic hormone containing cells. Characterization and localization of serum thymic factor in young mouse thymus studied by monoclonal antibodies. J Exp Med 156:628–633
Scollay R, Shortman K (1985) Identification of early stages of T lymphocyte development in the thymus cortex and medulla. J Immunol 134:3632–3642
Sokoloff L, Reivich M, Kennedy C, Des Rosiers MH, Patlak CS, Pettigrew KD, Sakurada O, Shinohara M (1977) The (14C)deoxy-glucose method for the measurement of local cerebral glucose utilization: theory procedure and normal values in the conscious and anesthetized albino rat. J Neurochem 28:897–916
Sokoloff L, Kennedy C, Smith CB (1983) Metabolic mapping of functional activity in the central nervous system by measurement of local glucose utilization with radioactive deoxyglucose. In: Björklund A, Hökfelt T (eds) Handbook of chemical neuroanatomy. Elsevier, Amsterdam, pp 416–440
Wulf J, Culter R (1975) Altered protein hypothesis of mammalian aging processes. I. Thermal stabilities of glucose-6-phosphate dehydrogenase in C57B1/6J mouse tissue. Exp Gerontol 10:101
Dedicated to Professor Dr. T.H. Schiebler on the occasion of his 65th birthday
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Warchol, J.B., Pilgrim, C., Ginda, W.J. et al. Autoradiographic studies on glucose utilization in individual zone of the rat thymus. Histochemistry 88, 571–573 (1988). https://doi.org/10.1007/BF00570326
- Public Health
- Stroma Cell
- Regional Difference
- Glucose Utilization