The use of the acetolysis reaction for the investigation of natural phospholipids
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1. When the acetolysis reaction is used to study the molecular types of natural phospholipids or to determine the bound glycerol, the possibility of a distortion of the results of analysis through the formation of by-products must be taken into account.
2. In order to prevent the formation of aldehyde acylals, the phospholipids undergoing analysis must first be freed from plasmalogens.
3. To prevent the formation of derivatives of propane-1,2-diol in the hydrolyzates of the acetolysis products of the phospholipids, the initial lipids must be freed from chloride ions.
KeywordsTotal Phospholipid Fatty Aldehyde Acid Methanolysis Alkaline Methanolysis Potassium Methoxide
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