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Integrated defective replication units of hepatitis B virus

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Abstract

Stable transformants of the human hepatoma cell line HepG2 were established that constitutively transcribe a DNA unit consisting of a stretch of hepatitis B virus DNA and of nonviral DNA conferring resistance to neomycin. Previously it had been shown that upon cotransfection of such transformants with replication-competent HBV DNA, transcripts of such units become reverse transcribed, demonstrating that DNA constructs can function as defective replication units. Transformed cell lines stably transcribing the defective replication units could be shown to use the transcriptional starts for the viral pregenome and for the large core antigen at a ratio of 9:1. Upon the induction of replicative processes in the transformed cells by transfection with replication-competent wild type (wt) DNA, defective pregenomes transcribed from the integrated state became included in the pool of replicating nucleic acids.

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References

  1. 1.

    GanemD. and VarmusH.E., Ann Rev Biochem56, 651–693, 1987.

  2. 2.

    YaginumaK., ShirakataY., KobayashiM., and KoikeK., Proc Natl Acad Sci USA84, 2678–2682, 1987.

  3. 3.

    SellsM.A., ChenM.-L., and AcsG., Proc Natl Acad Sci USA84, 1005–1009, 1987.

  4. 4.

    SchranzP., ZentgrafH., LoncarevicI.F., NiepmannM., and SchröderC.H., J Virol64, 1851–1854, 1990.

  5. 5.

    AdenD.P., FogelA., PlotkinS., DamjanovI., and KnowlesB., Nature282, 615–616, 1979.

  6. 6.

    GrahamF.L., VeldhuisenG., and WilkieN.M., Virology52, 456–467, 1973.

  7. 7.

    WillH., ReiserW., WeimerT., PfaffE., BüscherB., SprengelR., CattaneoR., and SchallerH., J Virol61, 904–911, 1987.

  8. 8.

    EndersG.H., GanemD., and VarmusH.E., J Virol61, 35–41, 1978.

  9. 9.

    FarzaH., HadchouelM., ScottoJ., TiollaisP., BabinetC., and PourcelC., J Virol62, 4144–4152, 1988.

  10. 10.

    GalibertF., MandartE., FitoussiF., TiollaisP., and CharnayP., Nature281, 646–650, 1979.

  11. 11.

    WillH., CattaneoR., KochH.G., DaraiG., and SchallerH., Nature299, 740–742, 1982.

  12. 12.

    LoncarevicI.F., SchranzP., ZentgrafH., LiangX.-H., HerrmannG., TangZ.-Y., and SchröderC.H., Virology174, 158–168, 1990.

  13. 13.

    RadziwillG., ZentgrafH., SchallerH., and BoschV., Virology163, 123–132, 1988.

  14. 14.

    NagayaT., NakamuraT., TokinoT., TsurimotoT., ImaiM., MayumiT., KaminoK., YamamuraK., and MatsubaraK., Genes & Develop1, 773–782, 1987.

  15. 15.

    OkamotoH., TsudaF., and MayumiM., Jpn J Exp Med57, 217–221, 1987.

  16. 16.

    WillH., KuhnC., CattaneoR., and SchallerH. In: MiwaM., et al., (eds.)Primary and Tertiary Structure of Nucleic Acids and Cancer Research, Jpn Sci Soc Press, Tokyo, 1982, pp. 237–247.

  17. 17.

    PasekM., GotoT., GilbertW., ZinkB., SchallerH., MacKayP., LeadbetterG., and MurrayK., Nature282, 575–579, 1979.

  18. 18.

    ChirgwinJ.J., PrzbylaA.E., MacDonaldR.J., and RutterW.J., Biochemistry18, 5294–5299, 1979.

  19. 19.

    McKnightS.L. and KingsburyR., Science217, 316–324, 1982.

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Correspondence to Hanswalter Zentgraf.

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Schranz, P., Zentgraf, H. & Schröder, C.H. Integrated defective replication units of hepatitis B virus. Virus Genes 4, 367–374 (1990). https://doi.org/10.1007/BF00570031

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Key words

  • defective hepatitis B virus
  • integrants
  • replication