Morphogenesis of the tail fiber of bacteriophage T4
III. Isolation of the gene 35-product and the genes 36, 37 and 38 product
Summary
The formation of the tail fiber of bacteriophage T4 is controlled by genes 34, 35, 36, 37, 38 and 57. The gene 35 product was partially purified by IRC-50 column chromatography and by ammonium sulfate precipitation. The genes 36-37-38 directing component was purified 570 fold using the method of salting in and out and a sucrose density gradient centrifugation.
Some characters of the purified components and the complementation reaction between these two components were investigated.
Keywords
Precipitation Sulfate Ammonium Chromatography Sucrose
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References
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© Springer-Verlag 1971