Acta Diabetologica

, Volume 33, Issue 2, pp 154–158 | Cite as

Effect of biotin on glucokinase activity, mRNA expression and insulin release in cultured beta-cells

  • P. Borboni
  • R. Magnaterra
  • R. A. Rabini
  • R. Staffolani
  • O. Porzio
  • G. Sesti
  • A. Fusco
  • L. Mazzanti
  • R. Lauro
  • L. N. J. L. Marlier


Biotin is known to influence hepatic glucokinase (GK) expression both at a transcriptional and at a translational level. The aim of the present paper was to investigate the effect of biotin on pancreatic GK. For this purpose, RIN 1046-38 cells were cultured in the presence of different biotin concentrations for different times; there-after, GK mRNA expression, GK activity and insulin release were studied. Results demonstrated that biotin has a biphasic effect on GK mRNA expression, being stimulatory after short-term treatment and inhibitory after long-term treatment. GK activity was increased after long-term treatment. Insulin release was not affected by biotin treatment. These data suggest that biotin may influence glucose metabolism also by acting directly at the level of beta-cells.

Key words

Glucokinase Pancreatic islets Biotin 


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • P. Borboni
    • 1
  • R. Magnaterra
    • 1
  • R. A. Rabini
    • 2
  • R. Staffolani
    • 3
  • O. Porzio
    • 1
  • G. Sesti
    • 1
  • A. Fusco
    • 1
  • L. Mazzanti
    • 3
  • R. Lauro
    • 1
  • L. N. J. L. Marlier
    • 1
  1. 1.Department of Internal MedicineUniversity of Rome “Tor Vergata”RomeItaly
  2. 2.Department of DiabetologyINRCAAnconaItaly
  3. 3.Institute of BiochemistryUniversity of AnconaAnconaItaly

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