Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Herpes simplex virus-encoded ribonucleotide reductase: Evidence for the dissociation/ reassociation of the holoenzyme

  • 29 Accesses

  • 3 Citations

Abstract

35S-labeled cells infected with herpes simplex virus type 1 (HSV-1), temperature-sensitive (ts) mutantts 1222 were used as a source of the large subunit of the viral ribonucleotide reductase (RR) to investigate the binding of the large (RR1) and small (RR2) subunits in the active enzyme. Mixing35S-labeled RR1 fromts 1222 with unlabeled RR1/RR2 complex from wild type (wt) infected cells resulted in the formation of a complex between35S-labeled RR1 and unlabeled RR2, indicating that the complex between the RR1 and RR2 subunits is dynamic and subunit dissociation/reassociation occurs during enzyme function. Similar results were obtained when unlabeled HSV-2 RR was substituted for HSV-1 RR, demonstrating that the holoenzyme can be formed from the large subunit of HSV-1 RR and the small subunit of HSV-2.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Averett D. R., Lubbers C., Elian G. B. and Spector T., J Biol Chem258, 981–9838, 1983.

  2. 2.

    Huszar D. and Bacchetti S., J Virol37, 580–588, 1981.

  3. 3.

    Ponce de Leon M., Eisenberg R. J. and Cohen G. H., J Gen Virol36, 163–173, 1977.

  4. 4.

    Cohen G. H., J Virol9, 408–418, 1972.

  5. 5.

    Cohen G. H., Factor M. N. and Ponce de Leon M. J Virol14, 20–25, 1974.

  6. 6.

    McLauchlan J. and Clements J. B. J Gen Virol64, 997–1006, 1983.

  7. 7.

    Frame M. C., Marsden H. S. and Dutia B. M. J Gen Virol66, 1581–1587, 1985.

  8. 8.

    Cohen E. A., Charron J., Perret J. and Langelier Y. J Gen Virol66, 733–745, 1985.

  9. 9.

    Ingermarson R. and Lankinen H., Virology156, 417–422, 1987.

  10. 10.

    Darling A. J., McKay E. M., Ingemarson R. and Preston V. G., Virus Genes2, 163–170, 1988.

  11. 11.

    Huang A., Jacobi G., Haj-Ahmad Y. and Bacchetti S., Virology163, 462–470, 1988.

  12. 12.

    Ingemarson R., Gräslund A., Darling A. and Thelander L., J Virol63, 3769–3776, 1989.

  13. 13.

    Dutia B. M., Frame M. C., Subak-Sharpe J. H., Clark W. N. and Marsden H. S. Nature (London)321, 439–441, 1986.

  14. 14.

    Cohen E. A., Gaudreau P., Brazeau P. and Langelier Y., Nature (London),321, 441–443, 1986.

  15. 15.

    Gaudreau P., Michaud J., Cohen E. A., Langelier Y. and Brazeau P. J Biol Chem262, 12413–12416, 1981.

  16. 16.

    McClements W., Yamanaka G., Garsky V., Perry H., Bacchetti S., Colonno R. and Stein R. B. Virology162, 270–273, 1988.

  17. 17.

    Sioberg B.-M., Karlsson M. and Jornvall H. J Biol Chem263, 9736–9743, 1987.

  18. 18.

    Paradis H., Gaudreau P., Brazeau P. and Langelier Y., J Biol Chem263, 16045–16050, 1988.

  19. 19.

    Bacchetti S., Evelegh M. J. and Muirhead B., J Virol57, 1177–1181, 1986.

  20. 20.

    Cohen E. A., Gaudreau P., Brazeau P. and Langelier Y., J Virol60, 1130–1133, 1986.

  21. 21.

    Draper K. G., Frink R. J. and Wagner E. K., J Virol43, 1123–1128, 1982.

  22. 22.

    Galloway D. A. and Swain M. A., J Virol49, 724–730, 1984.

  23. 23.

    McLauchlan J. and Clements J. B., EMBO J2, 1953–1961, 1983.

  24. 24.

    Dutia B. M., J Gen Virol64, 513–521, 1983.

  25. 25.

    Preston V. G., Palfreyman J. W. and Dutia B. M., J Gen Virol65, 1457–1466, 1984.

  26. 26.

    Nutter L. M., Grill S. P. and Cheng Y-C., Biochem Pharm34, 777–780, 1985.

  27. 27.

    Goldstein D. J. and Weller S. K., J Virol62, 196–205, 1988.

  28. 28.

    Goldstein D. J. and Weller S. K., J Virol62, 2970–2977, 1988.

  29. 29.

    Preston V. G., Darling A. J. and McDougall I., Virology167, 1988.

  30. 30.

    Cameron J. M., McDougall I., Marsden H. S., Preston V. G., Ryan D. M. and Subak-Sharpe J. H., J Gen Virol 2607–2612, 1988.

  31. 31.

    Chung T. D., Wymer J. P., Smith C. C., Kulka M. and Aureliar L., J Virol63, 3389–3398, 1989.

  32. 32.

    Darling A. J., Dutia B. M. and Marsden H. S., J Virol Meth180, 281–290, 1987.

  33. 33.

    Showalter S. D., Zweig M. and Hampar B., Infect Immun34, 684–692, 1981.

Download references

Author information

Additional information

Requests for reprints should be addressed to Allan J. Darling, The Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, Scotland.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Darling, A.J., McKay, E.M., Ingemarson, R. et al. Herpes simplex virus-encoded ribonucleotide reductase: Evidence for the dissociation/ reassociation of the holoenzyme. Virus Genes 3, 367–372 (1990). https://doi.org/10.1007/BF00569043

Download citation

Key words

  • HSV
  • ribonucleotide reductase
  • subunit dissociation
  • reassociation