Chemistry of Natural Compounds

, Volume 2, Issue 2, pp 105–108 | Cite as

A study of enzyme-substrate compounds

II. Preparation of acyl-L-valyl, D-valyl, and D-phenylalanyl derivatives of chymotrypsin
  • M. M. Botvinik
  • I. P. Kuranova
  • L. L. Ivanov
Article
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Summary

1. From the reaction of chymotrypsin with the NPE of CBZ-L- and CBZ-D-valine the enzyme-substrate compounds CBZ-L-valylchymotrypsin and CBZ-D-valylchymotrypsin have been isolated. The methyl esters of benzoyl-D-phenylalanine and of benzoyl-L-valine also form enzyme-substrate compounds with chymotrypsin, although to a smaller extent. The methyl ester of benzoyl-D-valine does not react with chymotrypsin.

2. Conditions for the isolation of a water-soluble enzyme-substrate compound have been found.

Keywords

Methyl Ester Benzoyl Chymotrypsin Sodium Hydrogen Carbonate Phenyl Ester 
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Copyright information

© The Faraday Press, Inc 1967

Authors and Affiliations

  • M. M. Botvinik
    • 1
  • I. P. Kuranova
    • 1
  • L. L. Ivanov
    • 1
  1. 1.Lomonosov Moscow State UniversityUSSR

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