A study of enzyme-substrate compounds
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1. From the reaction of chymotrypsin with the NPE of CBZ-L- and CBZ-D-valine the enzyme-substrate compounds CBZ-L-valylchymotrypsin and CBZ-D-valylchymotrypsin have been isolated. The methyl esters of benzoyl-D-phenylalanine and of benzoyl-L-valine also form enzyme-substrate compounds with chymotrypsin, although to a smaller extent. The methyl ester of benzoyl-D-valine does not react with chymotrypsin.
2. Conditions for the isolation of a water-soluble enzyme-substrate compound have been found.
KeywordsMethyl Ester Benzoyl Chymotrypsin Sodium Hydrogen Carbonate Phenyl Ester
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