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Synthesis of glycoproteins in the Golgi complex of the mouse gallbladder epithelium during fasting, refeeding, and gallstone formation

A light microscopic autoradiographic and quantitative electron microscopic study

Summary

The mouse gallbladder epithelium was studied with light microscopic autoradiography and quantitative electron microscopy during fasting, refeeding and experimental gallstone formation. To determine the intracellular pathway of glycoproteins, H3-galactose was injected at different time intervals into the mice. At 10, 25 and 40 min after an intraperitoneal injection the gallbladders were fixed and prepared for light microscopy. As early as 10 min after injection, label was observed in supranuclear cytoplasmic regions and at 25 min, an increased radioactivity was present throughout the apical cytoplasm. At 40 min, silver grains were mainly present at the cell surface. Autoradiographs processed 25 min after an intraperitoneal H3-galactose injection after fasting for 48 h showed decreased supranuclear and apical radioactivity. After refeeding (12 h) there was an enhanced activity in both these regions. Animals fed a lithogenic diet for one month showed a marked increase of radioactive label mainly in cells of crypts and invaginations of the mouse gallbladder mucosa.

Morphometric measurements of the Golgi apparatus revealed that deprivation of food significantly diminished the volume density of the Golgi apparatus. Refeeding the animals restored the volume density values to normal levels. In the course of gallstone formation there was a further significant increase in the volume density of the Golgi complexes as compared to controls.

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References

  1. Bouchier, I.A.D., Clamp, J.R.: Glycoproteins in human bile. Clin. chim. Acta35, 219–224 (1971)

  2. Inferrera, C., Ferlazzo, A.: Cytochemical observations in the electron microscope on the gall bladder epithelium. J. submicr. Cytol.6, 261–274 (1974)

  3. Kalckar, H.M.: Uridinediphospho galactose: metabolism, enzymology and biology. In: Advances in enzymology (F.F. Nord ed.), Vol. 20, pp. 111–134. New York: Interscience Publisher Inc. 1958

  4. Koga, A.: Electron microscopic observations on the mucous secretory activity of the human gallbladder epithelium. Z. Zellforsch.139, 463–471 (1973)

  5. Luciano, L., Reale, E., Wolpers, C.: Die Feinstruktur der Gallenblase und der Gallengänge. V. Histochemische Lokalisierung von Mukosubstanzen im menschlichen Gallenblasenepithel. Histochemistry38, 57–70 (1974)

  6. McGuire, E.T., Jourdian, G.W., Carlson, D.M., Roseman, S.: Incorporation of D-galactose into glycoproteins. J. biol. Chem.240, PC4112-PC4115 (1965)

  7. Neutra, M., Leblond, C.P.: Synthesis of carbohydrate of mucus in the Golgi complex as shown by electron microscope radioautography of goblet cells from rats injected with glucose-H3. J. Cell Biol.30, 119–136 (1966a)

  8. Neutra, M., Leblond, C.P.: Radioautographic comparison of the uptake of galactose-H3 and glucose-H3 in the Golgi region of various cells secreting glycoproteins or mucopolysaccharides. J. Cell Biol.30, 137–150 (1966b)

  9. Sarcione, E.J.: The initial subcellular site of incorporation of hexoses into liver protein. J. biol. Chem.239, 1686–1689 (1964)

  10. Schrager, J., Oates, M.D.G., Rosbottom, A.: The isolation and partial characterization of the principal biliary glycoprotein. Digestion6, 338–355 (1972)

  11. Tepperman, J., Cardwell, F.T., Tepperman, H.M.: Induction of gallstones in mice by feeding a cholesterol-cholic acid containing diet. Amer. J. Physiol.206, 628–634 (1964)

  12. Wahlin, T.: Effects of lithogenic diets on mouse gallbladder epithelium. A histochemical cytochemical and morphometric study. Virchows Arch. Abt. B (1976) (in print)

  13. Wahlin, T., Bloom, G.D., Carlsöö, B.: Histochemical observations with the light and electron microscope on the mucosubstances of the normal mouse gallbladder epithelial cells. Histochemistry42, 119–131 (1974)

  14. Wahlin, T., Bloom, G.D., Carlsöö, B., Rhodin, L.: Effects of fasting and refeeding on secretory granules of the mouse gallbladder epithelium. A quantitative electron microscopic study. Gastroenterology70, 353–358 (1976)

  15. Wahlin, T., Schiebler, T. H.: Zur Entwicklung des Gallenblasenepithels des Meerschweinchens. II. Electronenmikroskopische und enzymhistochemische Untersuchungen. Histochemistry44, 253–275 (1975)

  16. Weibel, E.R., Elias, H.: Introduction to stereological principles. In: Quantitative methods in morphology (E.R. Weibel and E. Elias eds.), pp. 89–98. Berlin-Heidelberg-New York: Springer 1967

  17. Weibel, E.R., Stäubli, W., Gnägi, H.R., Hess, F.A.: Correlated morphometric and biochemical studies on the liver cell. I. Morphometric model, stereological methods and normal morphometric data for rat liver. J. Cell Biol.42, 68–91 (1969)

  18. Werner, I.: Studies on glycoproteins from mucous epithelium and epithelial secretions. Acta Soc. Med. (Uppsala)58, 1–55 (1953)

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Wahlin, T. Synthesis of glycoproteins in the Golgi complex of the mouse gallbladder epithelium during fasting, refeeding, and gallstone formation. Histochemistry 51, 133–140 (1977). https://doi.org/10.1007/BF00567219

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Keywords

  • Intraperitoneal Injection
  • Golgi Apparatus
  • Volume Density
  • Golgi Complex
  • Intracellular Pathway