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Spectrophotometric determination of a bacterial lipopolysaccharide fromEscherichia coli 675

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Abstract

A method has been developed for the quantitative spectrophotometric determination of the lipopolysaccharides ofE. coli 675. The maximum absorption of the lipopoly-saccharides fromE. coli 675 (259 nm) coincides with the maximum absorption (259 nm) of a number of polysaccharides from other Gram-negative bacteria, which can be used to determine lipopolysaccharides in various media.

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Literature cited

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    J. Janda and E. Work, FEBS Letters,16, 340 (1971).

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    N. A. Karamian, J. Pharm. Sci.,66, 723 (1977).

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Additional information

All-Union Scientific-Research Institute of Pharmacy, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 599–601, September–October, 1979.

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Shchedrina, L.E., Brutko, L.I. Spectrophotometric determination of a bacterial lipopolysaccharide fromEscherichia coli 675. Chem Nat Compd 15, 521–523 (1979). https://doi.org/10.1007/BF00565914

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Keywords

  • Spectrophotometric Determination
  • Galacturonic Acid
  • Chromic Acid
  • Bacterial Origin
  • Neutral Monosaccharide