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Fluorometric determination of hydroflumethiazide in human plasma and urine after its oral administration

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Summary

A spectrofluorometric method for determination of hydroflumethiazide in human plasma and urine has been developed. The limit of detection was 10 ng/ml plasma and 100 ng/ml urine. The plasma concentration of hydroflumethiazide was determined for 9–11 hours and excretion in urine for 24–37 hrs after oral administration of about 1 mg/kg body weight to 7 subjects. Plasma half life in healthy subjects was 1.9–2.1 h, and 2.7–8.6 h in patients during the period 4–9 hrs after dosing. Cumulative excretion in urine was 67–79% of the dose during 31–37 hrs in 6 subjects; one patient with renal disease was found to excrete only 25.8% of dose during 24 hours. Renal clearance of hydroflumethiazide was higher in the healthy subjects (0.29–0.44 1 h−1 kg−1) than in the patients (0.040–0.15 l h−1 kg−1). Plasma half life of hydroflumethiazide was not closely correlated with renal clearance of the drug, which suggests that other factors may play a role in determining plasma half life.

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References

  1. 1.

    Blagg, C. R.: Hydroflumethiazide — a new oral diuretic. Lancet1959 II, 311–313

  2. 2.

    Dettli, L., Spring, P.: Vergleichende pharmakologische Prüfung eines neuen Salidiureticums (Cyclopenthiazid) am gesunden Menschen. Z. ges. exp. Med. exp. Chir.134, 310–322 (1960)

  3. 3.

    Young, D. S., Forrester, T. M., Morgan, T. N.: A comparison of the diuretic action of the chlorothiazide analogues. Lancet1959 II, 765–768

  4. 4.

    Matheson, N. A., Morgan, T. N.: Diuretic action of chlorothiazide. Lancet1958 I, 1195–1199

  5. 5.

    Ford, R. V., Moyer, J. H., Spurr, C. L.: Clinical and laboratory observations on chlorothiazide (Diuril). Arch. int. Med.100, 582–592 (1957)

  6. 6.

    Tapia, F. A.: Diuretic and antihypertensive effects of polythiazide. Curr. ther. Res.3, 365–377 (1961)

  7. 7.

    Ford, R. V.: Clinical pharmacological investigation of polythiazide, a potent oral diuretic agent. Curr. ther. Res.3, 320–329 (1961)

  8. 8.

    Hutcheon, D. E., Leonard, G. B.: Therapeutic efficacy of diuretics with different durations of action. J. Amer. med. Ass.185, 640–642 (1963)

  9. 9.

    Beevers, D. G., Harpur, J. E., Hamilton, M.: The long-term treatment of hypertension with thiazide diuretics. Postgrad. med. J.47, 639–643 (1971)

  10. 10.

    Maronde, R. F., Milgrom, M., Dickey, J. M.: Potassium loss with thiazide therapy. Amer. Heart. J.78, 16–21 (1969)

  11. 11.

    Skovbo, P., Bjerregaard, P., Hvidt, R., Jesting, E., Lorentzen, F., Hvidt, S.: Diuretika og hypokaliæmi. Ugeskr. Læg.134, 1043–1047 (1972)

  12. 12.

    Reutter, F., Schaub, F.: Harnsäurestoffwechsel und Salidiuretica. Dtsch. med. Wschr.89, 1101–1104 (1964)

  13. 13.

    Heimsoth, V., Harmann, F.: Untersuchungen zur Störung des Harnsäurestoffwechsels nach Saluretica-Verabreichung. Dtsch. med. Wschr.90, 1905–1908 (1965)

  14. 14.

    Roediger, P. M., Alden, J., Beardwood, D., Hutchison, J. C.: Benzothiadiazines and diabetes mellitus. II. Comparison of hyperglycemic effects of bendroflumethiazide and chlorothiazide in hypertensive patients with diabetes mellitus. Curr. ther. Res.6, 670–677 (1964)

  15. 15.

    Runyan, J. W.: Influence of thiazide diuretics on carbohydrate metabolism in patients with mild diabetes. New Engl. J. Med.267, 541–543 (1962)

  16. 16.

    Garceau, Y., Davis, J., Hasegawa, J.: Quantitative TLC procedure for determination of hydroflumethiazide in biological fluids. J. pharm. Sci.63, 1793–1795 (1974)

  17. 17.

    Vandenheuvel, W. J. A., Gruber, V. F., Walker, R. W., Wolf, F. J.: GLC analysis of hydrochlorothiazide in blood and plasma. J. pharm. Sci.64, 1309–1312 (1975)

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Brørs, O., Jacobsen, S. & Arnesen, E. Fluorometric determination of hydroflumethiazide in human plasma and urine after its oral administration. Eur J Clin Pharmacol 11, 149–154 (1977). https://doi.org/10.1007/BF00562907

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Key words

  • Hydroflumethiazide
  • spectrofluorometry
  • pharmacokinetics
  • plasma half life
  • renal excretion
  • renal disease