Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Adh expression in species of themulleri subgroup ofDrosophila

Abstract

The locus encoding the NAD-dependent alcohol dehydrogenase (alcohol:NAD oxidoreductase; EC 1.1.1.1) is duplicated in some species of the cactophilicmulleri subgroup ofDrosophila. Here we report a survey on the expression throughout development and in different tissues for this system, its regulation using interspecific hybrids, and the effects induced by 2-propanol in 10 species belonging to four different clusters of the abovementioned subgroup. Our results indicate different expression throughout development and in different tissues,cis regulation in hybrid ovaries,trans (−) regulation in hybrid adults, and different responses to 2-propanol.

This is a preview of subscription content, log in to check access.

References

  1. Anderson, S. M., and McDonald, J. F. (1981a). Effect of environmental alcohol on in vivo properties ofDrosophila alcohol dehydrogenase.Biochem. Genet. 19421.

  2. Anderson, S. M., and McDonald, J. F. (1981b). Changes in levels of alcohol dehydrogenase during development ofDrosophila melanogaster.Can. J. Genet. Cytol. 23305.

  3. Anderson, S. M., and McDonald, J. F. (1983). Biochemical and molecular analysis of naturally occurring Adh variants inDrosophila melanogaster.Proc. Natl. Acad. Sci. USA 804798.

  4. Ayala, F. J., Powell, J. R., Tracey, M. L., Mourao, A., and Perez-Salas, S. (1972). Enzyme variability in theDrosophila willistoni group. IV. Genetic variation in natural populations inDrosophila willistoni.Genetics 70113.

  5. Batterham, P., Gritz, E., Starmer, W. T., and Sullivan, D. T. (1983a). Biochemical characterization of the products of theAdh locus ofDrosophila mojavensis.Biochem. Genet. 21871.

  6. Batterham, P., Lovett, J. A., Starmer, W. T., and Sullivan, D. T. (1983b). Differential regulation of duplicate alcohol dehydrogenase genes inDrosophila mojavensis.Dev. Biol. 96346.

  7. Batterham, P., Chambers, G. K., Starmer, W. T., and Sullivan, D. T. (1984). Origin and expression of an alcohol dehydrogenase gene duplication in the genusDrosophila.Evolution 38644.

  8. Benyajati, C., Wang, N., Reddy, A., Weinberg E., and Sofer, W. (1980). Alcohol dehydrogenase inDrosophila: Isolation and characterization of messenger RNA and cDNA clone.Nucleic Acid Res 85649.

  9. Benyajati, C., Spoerel, N., Haymarle, H., and Ashburner, M. (1983). The messenger RNA for alcohol dehydrogenase inDrosophila melanogaster differs in its 5′ end in different developmental stages.Cell 33125.

  10. Caverner, D. R., and Clegg, M. T. (1978). Dynamics of correlated genetic systems. IV. Multi-locus effects of ethanol stress environments.Genetics 90629.

  11. David, J. (1959). Etude quantitative du developpement de laDrosophila elevee en milieu axenique.Bull. Biol. Fr. Belg. 93472.

  12. David, J. L., Bocquet, C., Arens, M., and Fouillet, P. (1976). Biological role of alcohol dehydrogenase in the tolerance ofDrosophila melanogaster to aliphatic alcohols: Utilization of an ADH-null mutant.Biochem. Genet. 14989.

  13. Dickinson, W. J. (1980a). Complexcis-acting regulatory genes demonstrated inDrosophila hybrids.Dev. Genet. 1229.

  14. Dickinson, W. J. (1980b). Tissue specificity of enzyme expression regulated by diffusible factors. Evidence fromDrosophila hybrids.Science 207995.

  15. Dickinson, W. J. (1983). Tissue-specific allelic isozyme patterns andcis-acting developmental regulators. In Ratazzi, M. C., Scandalios, J. G., and Witt, G. S. (eds.)Isozymes Vol. 9. Alan R. Liss, Inc., New York.

  16. Dickinson, W. J., and H. L. Carson, (1979). Regulation of tissue specificity of an enzyme by a cis-acting genetic element: Evidence from interspecificDrosophila hybrids.Proc. Natl. Acad. Sci. USA 764559.

  17. Fogleman, J. C. (1982). The role of volatiles in the ecology of cactophilicDrosophila. In Barker, J. F. S., and Starmer, W. T. (eds.),Ecological Genetics and Evolution Academic Press, Sydney, pp. 191–206.

  18. Fontdevila, A. (1980). Cuanto polimorfismo cromosomico se pierde en la colonization? In Reig, O. (ed.),Ecologia y Genetica de la Especiacion, Equiniccio, Universidad Simon Bolivar, Caracas.

  19. Fontdevila, A., Santos, M., and Gonzalez, R. (1980). Genotype-isopropanol interaction in the Adh locus ofDrosophila buzzatii.Experientia 36398.

  20. Harris, H., and Hopkinson, D. A. (1976).Handbook of Enzyme Electrophoresis in Human Genetics North-Holland, Amsterdam.

  21. Hedrick, P. W., and McDonald, J. F. (1980). Regulatory gene adaptation: An evolutionary model.Heredity 4585.

  22. Heed, W. B. (1978). Ecology and genetics of Sonoran DesertDrosophila. In Brussard, P. F. (ed.),Ecological Genetics: The Interface Springer-Verlag, New York, pp. 109–126.

  23. Heed, W. B. (1982). The origin ofDrosophila in the Sonoran Desert. In Barker, J. S. F., and Starmer, W. T. (eds.),Ecological Genetics and Evolution Academic Press, Syndey, pp. 65–80.

  24. Kreitman, M. (1983). Nucleotide polymorphism at the alcohol dehydrogenase locus ofDrosophila melanogaster.Nature 304412.

  25. MacIntyre, R. J. (1972). Studies of enzyme evolution by subunit hybridization. In LeCam, L. M., Neyman, J., and Scott, E. C. (eds.),Proceedings of the Sixth Berkeley Symposium on Mathematical Statistics and Probability, Vol. V University of California Press, Berkeley, pp. 119–154.

  26. McDonald, J. F., and Ayala, F. J. (1978). Genetic and biochemical basis of enzyme activity variation in natural populations. I. Alcohol dehydrogenase inDrosophila melanogaster.Genetics 89371.

  27. Mills, L. E., Batterham, P., Alegre, J., Starmer, W. T., and Sullivan, D. T. (1986). Molecular genetic characterization of a locus that contains duplicateAdh genes inDrosophila mojavensis and related species.Genetics 112871.

  28. Mulley, J. C. (1975).Isozyme Variation in Drosophila buzzatii, M.Sc. University of Sydney, Sydney.

  29. Oakeshott, J. G., Chambers, G. K., East, P. D., Gibson, J. B., and Barker, J. S. F. (1982). Evidence for a genetic duplication involving alcohol dehydrogenase genes inDrosophila buzzatii and related species.Austral. J. Biol. Sci. 3573.

  30. Papel, I., Henderson, M., Van Herewege, J., David, J., and Sofer, W. (1979).Drosophila alcohol dehydrogenase: Effects of exposing adults to acetone.Biochem. Genet. 17553.

  31. Poulik, M. D. (1957). Starch gel electrophoresis in discontinuous system buffers.Nature 1801477.

  32. Rabinow L., and Dickinson, W. J. (1986). Complexcis-acting regulators and locus structure ofDrosophila tissue-specific ADH variants.Genetics 112523.

  33. Sanchez, A. (1986). Ph.D. dissertation, Universitat Autonoma de Barcelona, Barcelona.

  34. Schwartz, M., and Sofer, W. (1976). Diet-induced alterations in distribution of multiple forms of alcohol dehydrogenase inDrosophila.Nature 263129.

  35. Schwartz, M., O'Donell, J., and Sofer, W. (1979). Origin of the multiple forms of alcohol dehydrogenase fromDrosophila melanogaster.Arch. Biochem. Biophys. 194365.

  36. Van Delden, W., Kamping, A., and van Dijk, H. (1975). Selection at the alcohol dehydrogenase locus inDrosophila melanogaster.Experientia 31418.

  37. Valentine, J. W., and Campbell, C. A. (1975). Genetic regulation and the fosil record.Am. Sci. 63673.

  38. Wasserman, M. (1982). Therepleta species group. In Ashburner, M., Carson, H. L., and Thompson, J. N., Jr. (eds.),The Genetics and Biology of Drosophila, Vol 3b Academic Press, London, pp. 61–139.

  39. Wilson, A. C. (1976). Gene regulation in evolution. In Ayala, F. J. (ed.),Molecular Evolution Sinauer Press, Sunderland, Mass., pp. 225–234.

Download references

Author information

Additional information

This research was supported by Grant 0910/81 from CAICYT to A.F. and a fellowship from CIRIT to J.A.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Alberola, J., Sanchez, A. & Fontdevila, A. Adh expression in species of themulleri subgroup ofDrosophila . Biochem Genet 25, 729–738 (1987). https://doi.org/10.1007/BF00556215

Download citation

Key words

  • mulleri subgroup ofDrosophila
  • alcohol dehydrogenase
  • developmental expression
  • regulation
  • 2-propanol