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The identification of nuclear and mitochondrial genes by sequencing randomly chosen clones from a marsupial mammary gland cDNA library


To increase the number of genes that can be mapped to the genome of the tammar wallaby (Macropus eugenii), we sequenced 100 randomly chosen clones from a mammary gland cDNA library. Provisional identifications were made of seven nuclear genes and one mitochondrial gene encoding two caseins, β-galactosidase, acetyl-coenzyme A synthetase, lipoprotein lipase, inorganic pyrophosphatase, an ATP-dependent RNA helicase, and cytochromec oxidase I. Highly conserved genes, such as that encoding acetyl-coenzyme A synthetase, were easily identified even from cross-kingdom matches. Genes which are highly divergent, however, such as those encoding themature casein peptides, could not be aligned with homologues in the databases. Even in an organ where there is high mRNA species redundancy, the sequence characterization of expressed sequence tags provides a rapid means of gene identification for mapping purposes.

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  1. Adams, M. D., Kelley, J. M., Gocayne, J. D., Dubnick, M., Polymeropoulos, M. H., Xiao, H., Merril, C. R., Wu, A., Olde, B., Moreno, R., Kerlavage, A. R., McCombie, W. R., and Venter, C. J. (1991). Complementary DNA sequencing: Expressed sequence tags and human genome project.Science 2521651.

  2. Adams, M. D., Dubnick, M., Kerlavage, A. R., Moreno, R., Kelley, J. M., Utterback, T. R., Nagle, J. W., Fields, C., and Venter, C. J. (1992). Sequence identification of 2,375 human brain genes.Nature 355632.

  3. Adams, M. D., Kerlavage, A. R., Fields, C., and Venter, C. J. (1993). 3,400 new expressed sequence tags identify diversity of transcripts in human brain.Nat. Genet. 4256.

  4. Air, G. M., Thompson, E. O., Richardson, B. J., and Sharman, G. B. (1971). Amino-acid sequence of kangaroo myoglobin and haemoglobin and the date of marsupial-eutherian divergence.Nature 229391.

  5. Altshul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990). Basic local alignment search tool.J. Mol. Biol. 215403.

  6. Archer, M. (1984). Origins and early radiations of mammals. In Archer, M., and Clayton, G. (eds.),Vertebrate Zoogeography and Evolution in Australia Hesperian Press, Carlisle, Western Australia, p. 477.

  7. Bonsing, J., and Mackinlay, A. G. (1987). Recent studies on nucleotide sequences encoding the caseins.J. Dairy Res. 54447.

  8. Collet, C., and Joseph, R. (1993). A novel member of the lipocalin superfamily: Tammar wallaby late-lactation protein.Biochim. Biophys. Acta 1167219.

  9. Collet, C., Joseph, R., and Nicholas, K. R. (1989). Molecular cloning and characterisation of a novel marsupial milk protein gene.Biochem. Biophys. Res. Commun. 1641380.

  10. Collet, C., Joseph, R., and Nicholas, K. R. (1990). Cloning, cDNA analysis and prolactin-dependent expression of a marsupial α-lactalbumin.Reprod. Fert. Dev. 2693.

  11. Collet, C., Joseph, R., and Nicholas, K. R. (1991). A marsupial β-lactoglobulin: Characterization and prolactin-dependent expression.J. Mol. Endocrinol. 69.

  12. Graves, J. A. M. (1987). The evolution of mammalian sex-chromosomes and dosage compensation: Clues from marsupials and monotremes.Trends Genet. 3252.

  13. Groenen, M. A. M., Dijkhof, R. J. M., Verstege, A. J. M., and van der Poel, J. J. (1993). The complete sequence of the gene encoding bovine αs2-casein.Gene 123187.

  14. Hinds, L. A., Poole, W. E., Tyndale-Biscoe, C. H., van Oorschot, R. A. H., and Cooper, D. W. (1990). Reproductive biology and the potential for genetic studies in the tammar wallaby (Macropus eugenii).Aust. J. Zool. 37223.

  15. Hoog, C. (1991). Isolation of a large number of novel mammalian genes by a differential cDNA library screening strategy.Nucl. Acids Res. 196123.

  16. Khan, A. S., Wilcox, A. S., Polymeropoulos, M. H., Hopkins, J. A., Stevens, T. J., Robinson, M., Orpana, A. K., and Sikela, J. M. (1992). Single-pass sequencing and the physical and genetic mapping of human brain cDNAs.Nat. Genet. 2180.

  17. McCombie, W. R., Adams, M. D., Kelley, J. M., Fitzgerald, M. G., Utterback, T. R., Khan, M., Dubnick, M., Kerlavage, A. R., Venter, C. J., and Fields, C. (1992).Caenorhabditis elegens expressed sequence tags identify gene families potential disease gene homologues.Nat. Genet. 1124.

  18. McKenzie, L. M., Collet, C., and Cooper, D. W. (1993). Use of a sub-species cross for efficient development of a linkage map for a marsupial mammal, the tammar wallaby (Macropus eugenii).Cytogenet. Cell Genet. 64264.

  19. Okubo, K., Hori, N., Matoba, R., Niiyama, T., Fukushima, A., Kojima, Y., and Matsubara, K. (1992). Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression.Nat. Genet. 2173.

  20. Pearson, W. R., and Lipman, D. J. (1988). Improved tools for biological sequence comparison.Proc. Natl. Acad. Sci. USA 852444.

  21. Sanger, F., Nicklen, S., and Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors.Proc. Natl. Acad. Sci. USA 745463.

  22. Tyndale-Biscoe, C. H., and Janssens, P. A. (1988).The Developing Marsupial: Models for Biomedical Research Springer-Verlag, Berlin.

  23. Tyndale-Biscoe, C. H., and Renfree, M. (1987).Reproductive Physiology of Marsupials Cambridge University Press, Cambridge.

  24. Waterston, R., Martin, C., Craxton, M., Huynh, C., Coulson, A., Hillier, L., Durbin, R., Green, P., Shownkeen, R., Halloran, N., Metzstein, M., Hawkins, T., Wilson, R., Berks, M., Du, Z., Thomas, K., Thierry-Mieg, J., and Sulston, J. (1992). A survey of expressed genes inCaenorhabditis elegans.Nat. Genet. 1114.

  25. Woodlee, G. L., Gooley, A. A., Collet, C., and Cooper, D. W. (1994). Evolution of marsupial milk proteins: Evidence for the tammar wallaby for the origin of late-lactation protein from β-lactoglobulin.J. Hered. 84460.

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Correspondence to C. Collet.

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Collet, C., Joseph, R. The identification of nuclear and mitochondrial genes by sequencing randomly chosen clones from a marsupial mammary gland cDNA library. Biochem Genet 32, 181–190 (1994). https://doi.org/10.1007/BF00554621

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Key words

  • marsupial
  • cDNA clones
  • expressed sequence tags
  • gene identification