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Isoenzymes related to flight activity inTetragonisca angustula (Hymenoptera:Apidae:Meliponinae): Evidence of postranslational modification of the hexokinase and detection of new glycerol-3-phosphate dehydrogenase variants

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Abstract

To detect genetic markers, electrophoretic analyses were carried out onTetragonisca angustula, a stingless bee well adapted to its wide area of occurrence, which is becoming economically important because of the quality of its honey. Polymorphism of the enzyme hexokinase was detected and it was demonstrated that, although it presents two bands in meliponines, this enzyme is under the control of a single locus whose product probably undergoes postranslational modification. The more common allele occurs in the twoTetragonisca subspecies studied here, but the other seems to be characteristic of only one subspecies. Four variants involving the two glycerol-3-phosphate dehydrogenase loci are also described. These two enzymes share the property of presenting two electrophoretic bands, one of which is characteristic of actively flying adult thoraces.

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Correspondence to Eucleia P. B. Contel.

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Castanheira, E.B., Contel, E.P.B. Isoenzymes related to flight activity inTetragonisca angustula (Hymenoptera:Apidae:Meliponinae): Evidence of postranslational modification of the hexokinase and detection of new glycerol-3-phosphate dehydrogenase variants. Biochem Genet 33, 365–375 (1995). https://doi.org/10.1007/BF00554595

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Key words

  • stingless bees
  • flight metabolism
  • isoenzymes
  • hexokinase
  • glycerol-3-phosphate dehydrogenase