Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Isozyme relative mobility (R m ) changes related to leaf position; apparently smoothR m trends and some implications

  • 12 Accesses

  • 1 Citations


Relative mobilities (R m 's) of peroxidase and acid phosphatase isozymes were examined in leaves of flax (Linum usitatissimum L.). The leaves were sampled from four equidistantly spaced positions from main stem base to apex in various genotypes.R m 's for the two slowest-migrating isozymes of each enzyme system changed in a simple, coherent fashion in leaves from stem bases toward apices. TheR m trends up the stem seen in two highly branched flax types were somewhat different from those in two sparsely branched types. The coherentR m trends in the four types, suggesting a smooth continuum and a potentially large number of slightly different forms of these isozymes, are discussed in relation to other data for suchR m trends. In the study reported here, both enzyme systems behaved similarly. This fact and the simple Mendelian genetical system with no codominance controllingR m 's in flax peroxidases and acid phosphatases suggest posttranscriptional or posttranslational modifications as plausible mechanisms underlying the numerous, presumably small molecular changes generating the small, consistent changes inR m 's.

This is a preview of subscription content, log in to check access.


  1. Durrant, A. (1971). The environmental induction of heritable change inLinum.Heredity 1727.

  2. Fieldes, M. A., and Tyson, H. (1972). Activity and relative mobility of peroxidase isozymes in genotrophs and genotypes of flax (Linum usitatissimum L.).Can. J. Genet. Cytol. 14625.

  3. Fieldes, M. A., and Tyson, H. (1973). Activity and relative mobility of peroxidase and esterase isozymes of flax (Linum usitatissimum) genotrophs. I. Developing main stems.Can. J. Genet. Cytol. 15731.

  4. Fieldes, M. A., and Tyson, H. (1983). Molecular weight differences in acid phosphatases of stem homogenates from L and S flax genotrophs.Biochem. Gen. 21391.

  5. Fieldes, M. A., and Tyson, H. (1984). Possible post-translational modification, and its genetic control, in flax genotroph isozymes.Biochem. Genet. 2299.

  6. Fieldes, M. A., Bashour, N., Deal, C. L., and Tyson, H. (1976a). Isolation of peroxidase isozymes from two flax genotypes by column chromatography.Can. J. Bot. 541180.

  7. Fieldes, M. A., Tyson, H., and Bashour, N. (1976b). Relative mobility shifts in anionic peroxidase isozymes between stem base and apex of flax genotrophs.Phytochemistry 15247.

  8. Fieldes, M. A., Deal, C. L., and Tyson, H. (1977). Preliminary characterisation of peroxidase isozymes isolated from two flax genotrophs.Can. J. Bot. 551465.

  9. Fieldes, M. A., Starobin, J., and Tyson, H. (1984). Visualisation of both peroxidase and acid phosphatase isozymes from flax (Linum) on the same gels.Anal. Biochem. 137146.

  10. Finnerty, V., and Johnson, G. (1979). Post-translational modification as a potential explanation of high levels of enzyme polymorphism: Xanthine dehydrogenase and alcohol oxidase inDrosophila melanogaster.Genetics 91695.

  11. Harry, D. (1983). Identification of a locus modifying the electrophoretic mobility of malate dehydrogenase isozymes in incense cedar (Calocedrus decurrens), and its implications for population studies.Biochem. Genet. 21417.

  12. Hemmings, B. A., Zubenko, G. S., Haslik, A., and Jones, E. W. (1981). Mutant defective in processing of an enzyme located in the lysosome-like vacuole ofSaccharomyces cerevisiae.Proc. Natl. Acad. Sci. USA 78435.

  13. Koehn, R. K. (1978). Physiology and biochemistry of enzyme variation: The interface of ecology and population genetics. In Brussard, P. F. (ed.),Ecological Genetics: The Interface Springer-Verlag, New York, pp. 51–72.

  14. Maclean, N. (1976).Control of Gene Expression Academic Press, New York, p. 46.

  15. Mastonbroek, O., Hogeweg, P., Heringa, J., Niemann, G. J., Van Nigtevecht, G., and Van Brederode, J. (1984). Isozyme variation inSilene pratensis: A response to different environments.Biochem. System. Ecol. 1229.

  16. Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bio-assays with tobacco tissue.Physiol. Plant. 15473.

  17. Perkins, J. M., Eglinton, E. G., and Jinks, J. L. (1971). The nature of the inheritance of the permanently induced changes inNicotiana rustica.Heredity 27441.

  18. Sokal, R. R., and Rohlf, F. J. (1981).Biometry Freeman, San Francisco, p. 179.

  19. Tyson, H., Taylor, S. A., and Fieldes, M. A. (1978). Segregation of the environmentally induced relative mobility shifts in flax genotroph peroxidase isozymes.Heredity 40281.

  20. Van den Berg, B. M., Bianchi, F., and Wijsman, H. J. W. (1983). Genetics of the peroxidase isoenzymes inPetunia. 5. Differential temporal expression of prxA alleles.Theoret. Appl. Genet. 651.

  21. Welinder, K. G. (1976). Covalent structure of the glycoprotein horseradish peroxidase (EC Lett. 7219.

  22. Williams, D. B., and Lennarz, W. J. (1984). Control of asparagine-linked oligosaccharide chain processing: Studies on bovine pancreatic ribonuclease B—an in vitro system for the processing of exogenous glycoproteins.J. Biol. Chem. 2595105.

  23. Williams, R. O., Young, J. R., and Majiwa, P. A. O. (1979). Genomic rearrangements correlated with antigenic variation inTrypanosoma brucei.Nature (Lond.) 282847.

Download references

Author information

Additional information

The financial assistance of the Natural Sciences and Engineering Council of Canada is acknowledged with thanks.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Tyson, H., Fieldes, M.A., Cheung, C. et al. Isozyme relative mobility (R m ) changes related to leaf position; apparently smoothR m trends and some implications. Biochem Genet 23, 641–654 (1985). https://doi.org/10.1007/BF00554080

Download citation

Key words

  • isozymeR m
  • leaf position andR m