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Oecologia

, Volume 51, Issue 2, pp 191–198 | Cite as

Genetic variation at the alcohol dehydrogenase locus in Drosophila melanogaster in relation to environmental variation: Ethanol levels in breeding sites and allozyme frequencies

  • J. B. Gibson
  • T. W. May
  • A. V. Wilks
Article

Summary

Ethanol levels in Drosophila breeding sites in seepages of unfortified wine inside wineries have been found to be similar to those in many decaying fruits and vegetables. Fortified wine seepages inside wineries have ethanol levels on average three times as high as other breeding sites. However there was no evidence that this variation in ethanol levels was associated with differences in Adh F frequencies in D. melanogaster at sites either within wineries or outside wineries. D. simulans was at lower frequencies at sites inside wineries compared to sites outside although this difference may not be related to ethanol levels. It is concluded that adaptation to natural levels of environmental ethanol by D. melanogaster does not necessarily modify Adh frequencies.

Keywords

Alcohol Genetic Variation Environmental Variation Alcohol Dehydrogenase Breeding Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Amerine MA, Kunkee RE (1968) Microbiology of Winemaking. Ann Rev Microbiol 22:323–358Google Scholar
  2. Anderson PR (1981) Geographic clines and climatic associations of Adh and α-Gpdh gene frequencies in Drosophila melanogaster. In: JB Gibson and JG Oakeshott (eds), Genetic Studies of Drosophila Populations. Australian National University, Canberra p 237–250Google Scholar
  3. Briscoe DA, Robertson A, Malpica J (1975) Dominance at Adh locus in response of adult Drosophila melanogaster to environmental alcohol. Nature 255:248–249Google Scholar
  4. Brien JF, Loomis CW (1978) Gas-liquid chromatographic determination of ethanol and acetaldehyde in blood. Clin Chim Acta 87:175–180Google Scholar
  5. Cavener D (1979) Preference for ethanol in Drosophila melanogaster associated with the alcohol dehydrogenase polymorphism. Behaviour Genetics 9:359–365Google Scholar
  6. Chambers GK, Wilks AV, Gibson JB (1981) An electrophoretically cryptic alcohol dehydrogenase variant in Drosophila melanogaster. III. Biochemical properties and comparison with the common enzyme forms. Aust J Biol Sci in pressGoogle Scholar
  7. Clarke B (1975) The contribution of ecological genetics to evolutionary theory: detecting the direct effect of natural selection on particular polymorphic loci. Genetics 79:101–113Google Scholar
  8. David JR (1977) Signification d'un polymorphisme enzymatique: la déshydrogénase alcoolique chez Drosophila melanogaster. Ann Biol 16:451–472Google Scholar
  9. Fuyama Y (1976) Behaviour genetics of olfactory responses in Drosophila. I. Olfactometry and strain differences in Drosophila melanogaster. Behaviour Genetics 6:407–420Google Scholar
  10. Fuyama Y (1978) Behaviour genetics of olfactory responses in Drosophila. II. An odorant-specific variant in a natural population of Drosophila melanogaster. Behaviour Genetics 8:399–414Google Scholar
  11. Gelfand LJ, McDonald JF (1980) Relationship between ADH activity and behavioural response to environmental alcohol in Drosophila. Behaviour Genetics 10:237–248Google Scholar
  12. Gibson JB (1970) Enzyme flexibility in Drosophila melanogaster. Nature 227:959–961Google Scholar
  13. Gibson JB (1972) Differences in the number of molecules produced by two allelic electrophoretic enzyme variants in D. melanogaster. Experientia 28:975–976Google Scholar
  14. Gibson JB, Lewis N, Adena MA, Wilson SR (1979) Selection for ethanol tolerance in two populations of Drosophila melanogaster segregating alcohol dehydrogenase allozymes. Aust J Biol Sci 32:387–398Google Scholar
  15. Hedrick PW, Ginevan MR, Swing EP (1976) Genetic polymorphism in heterogeneous environments. Ann Rev Ecol Syst 7:1–32Google Scholar
  16. Hickey DA, McLean MD (1980) Selection for ethanol tolerance and Adh allozymes in natural populations of Drosophila melanogaster. Genet Res 36:11–15Google Scholar
  17. Koehn RK (1978) Physiology and Biochemistry of Enzyme Variation: The Interface of Ecology and Population Genetics. In: PF Brussard (ed), Ecological Genetics: The Interface. Springer, Berlin Heidelberg New York, p 51–72Google Scholar
  18. Lewis N, Gibson JB (1978) Enzyme protein amount variation in natural populations. Biochem Genet 16:159–170Google Scholar
  19. May TW (1980) The distribution of species and phenotypes in an ecological gradient: Field and laboratory studies of Drosophila melanogaster and D. simulans. Unpublished Thesis, The University of MelbourneGoogle Scholar
  20. Marks RW, Brittnascher JG, McDonald JF, Prout T, Ayala FJ (1980) Wineries, Drosophila, Alcohol, and Adh. Oecologia (Berl) 47:141–144Google Scholar
  21. McKenzie JA (1974) The distribution of vineyard populations of Drosophila melanogaster and Drosophila simulans during vintage and non-vintage periods. Oecologia (Berl) 15:1–16Google Scholar
  22. McKenzie JA (1980) An ecological study of the alcohol dehydrogenase (Adh) polymorphism of Drosophila melanogaster. Aust J Zool 28:709–716Google Scholar
  23. McKenzie JA, McKechnie SW (1978) Ethanol tolerance and the Adh polymorphism in a natural population of Drosophila melanogaster. Nature 272:75–76Google Scholar
  24. McKenzie JA, McKechnie SW (1979) A comparative study of resource utilization in natural populations of Drosophila melanogaster and D. simulans. Oecologia (Berl) 40:299–309Google Scholar
  25. McKenzie JA, McKechnie SW (1981) The alcohol dehydrogenase polymorphism in a vineyard cellar population of Drosophila melanogaster. In: JB Gibson and JG Oakeshott (eds), Genetic Studies of Drosophila Populations. Australian National University, Canberra p 201–215Google Scholar
  26. McKenzie JA, Parsons PA (1972) Alcohol tolerance: An ecological parameter in the relative success of Drosophila melanogaster and Drosophila simulans. Oecologia (Berl) 10:373–388Google Scholar
  27. McKenzie JA, Parson PA (1974) Microdifferentiation in a natural population of Drosophila melanogaster to alcohol in the environment. Genetics 77:385–394Google Scholar
  28. Morgan P (1975) Selection acting directly on an enzyme polymorphism. Heredity 34:124–127Google Scholar
  29. Nevo E (1978) Genetic variation in natural populations: patterns and theory. Theor Pop Biol 13:121–177Google Scholar
  30. Oakeshott JG (1976) Selection at the alcohol dehydrogenase locus in Drosophila melanogaster imposed by environmental ethanol. Genet Res 26:265–274Google Scholar
  31. Oakeshott JG, Gibson JB (1981) Is there selection by environmental ethanol on the alcohol dehydrogenase locus in Drosophila melanogaster? In: JB Gibson and JG Oakeshott (eds), Genetic Studies of Drosophila Populations. Australian National University, Canberra p 103–120Google Scholar
  32. Oakeshott JG, Gibson JB, Anderson PR, Champ A (1980) Opposing modes of selection on the alcohol dehydrogenase locus in Drosophila melanogaster. Aust J Biol Sci 33:105–114Google Scholar
  33. Oakeshott JG, Gibson JB, Anderson PR, Knibb WR, Anderson DG, Chambers GK (1981) Alcohol dehydrogenase and glycerol-3-phosphate dehydrogenase clines in Drosophila melanogaster on three continents. Evolution in pressGoogle Scholar
  34. Parsons PA (1977) Larval reaction to alcohol as an indicator of resource utilization differences between Drosophila melanogaster and Drosophila simulans. Oecologia (Berl) 30:141–146Google Scholar
  35. Parsons PA (1980) Larval responses to environmental ethanol in Drosophila melanogaster: Variation within and among populations. Behaviour Genetics 10:183–190Google Scholar
  36. Parsons PA, King SB (1977) Ethanol larval discrimination between two Drosophila sibling species. Experientia 33:898–899Google Scholar
  37. Parsons PA, Stanley SM, Spence GE (1979) Environmental ethanol at low concentrations: Longevity and development in the sibling species Drosophila melanogaster and D. simulans. Aust J Zool 27:747–754Google Scholar
  38. Parsons PA, Stanley SM (1981) Comparative effects of environmental ethanol on Drosophila melanogaster and D. simulans adults, including geographic differences in D. melanogaster. In: JB Gibson and JG Oakeshott (eds), Genetic Studies of Drosophila Populations. Australian National University, Canberra p 47–57Google Scholar
  39. Powell JR (1975) Protein variation in natural populations of animals. Evolutionary Biology 8:79–119Google Scholar
  40. Richmond RC, Gerking JL (1979) Oviposition site preference in Drosophila. Behaviour Genetics 9:233–241Google Scholar
  41. Taylor CE, Powell JR (1978) Habitat choice in natural populations of Drosophila. Oecologia (Berl) 37:69–75Google Scholar
  42. Thompson JN, Kaiser TN (1977) Selection acting upon slow-migrating ADH alleles differing in enzyme activity. Heredity 38:191–195Google Scholar
  43. Vigue CL, Johnson FM (1973) Isozyme variability in species of the genus Drosophila. VI Frequency-property-environment relationship of allelic alcohol dehydrogenases in D. melanogaster. Biochem Genet 9:213–227Google Scholar
  44. Wilks AV, Gibson JB, Oakeshott JG, Chambers GK (1980) An electrophoretically cryptic alcohol dehydrogenase variant in Drosophila melanogaster. II. Post-electrophoresis heat-treatment screening of natural populations. Aust J Biol Sci 33:575–585Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • J. B. Gibson
    • 1
  • T. W. May
    • 1
  • A. V. Wilks
    • 1
  1. 1.Department of Population Biology, Research School of Biological SciencesThe Australian National UniversityCanberra CityAustralia

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