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Mammalian Genome

, Volume 6, Issue 11, pp 809–815 | Cite as

Genetic and physical mapping of the natural resistance-associated macrophage protein 1 (NRAMP1) in chicken

  • J. Hu
  • N. Bumstead
  • D. Burke
  • F. A. Ponce de León
  • E. Skamene
  • P. Gros
  • D. Malo
Original Contributions

Abstract

The chicken natural resistance-associated macrophage protein 1 (NRAMP1) gene has been mapped by linkage analysis by use of a reference panel to develop the chicken molecular genetic linkage map and by fluorescence in situ hybridization. The chicken homolog of the murine Nramp1 gene was mapped to a linkage group located on Chromosome (Chr) 7q13, which includes three genes (CD28, NDUSF1, and EF1B) that have previously been mapped either to mouse Chr 1 or to human Chr 2q. Physical mapping by pulsed-field gel electrophoresis revealed that NRAMP1 is tightly linked to the villin gene and that the genomic organization (gene order and presence of CpG islands) of the chromosomal region carrying NRAMP1 is well conserved between the chicken and mammalian genomes. The regions on mouse Chr 1, human Chr 2q, and chicken Chr 7q that encompass NRAMP1 represent large conserved chromosomal segments between the mammalian and avian genomes. The chromosome mapping of the chicken NRAMP1 gene is a first step in determining its possible role in differential susceptibility to salmonellosis in this species.

Keywords

Linkage Group Physical Mapping Linkage Analysis Gene Order Genomic Organization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1995

Authors and Affiliations

  • J. Hu
    • 1
  • N. Bumstead
    • 4
  • D. Burke
    • 5
  • F. A. Ponce de León
    • 5
  • E. Skamene
    • 1
  • P. Gros
    • 2
  • D. Malo
    • 1
    • 3
  1. 1.Department of MedicineMcGill UniversityMontrealCanada
  2. 2.Department of BiochemistryMcGill UniversityMontrealCanada
  3. 3.Department of Human GeneticsMcGill UniversityMontrealCanada
  4. 4.Institute for Animal HealthComptonEngland, UK
  5. 5.Department of Veterinary and Animal ScienceUniversity of MassachusettsAmherstUSA

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