The effect of staphylococcal α-toxin on blood platelets
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Summary
Staphylococcal α-toxin incubated with rabbit blood platelets induces an early blood platelet aggregation with subsequent release of 5-HT. The phenomenon of platelet aggregation is accompanied by release of ATPase activity into the supernatant. An electron microscope examination of platelets after the addition of toxin revealed characteristic morphological changes.
Key-Words
Staphylococcal α-toxin Blood Platelets Aggregation 5-hydroxytryptamineSchlüsselwörter
Staphylokokkentoxin α Blutplättchen Aggregation 5 HydroxytryptaminPreview
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References
- Bernheimer, A. W., and L. L. Schwartz: Effect of Staphylococcal and other bacterial toxins on platelets in vitro. J. Path. Bact. 89, 209 (1965).Google Scholar
- Born, G. V. R.: Aggregation of blood platelets by ADP and its reversal. Nature (Lond.) 194, 927 (1962).Google Scholar
- ——, and M. J. Cross: The aggregation of blood platelets. J. Physiol. (Lond.) 168, 178 (1963).Google Scholar
- Brown, D. A., and J. P. Quilliam: Some effects of Staphylococcal toxin on isolated mammalian smooth muscle preparation. Brit. J. Pharmacol. 25, 781 (1965).Google Scholar
- Hovig, T.: The effect of various enzymes on the ultrastructure, aggregation, and clot retraction ability of rabbit blood platelets. Thrombos. Diathes. haemorrh. (Stuttg.) 13, 84 (1965).Google Scholar
- Jeljaszewicz, J., S. Niewiarowski, A. Poplawski, and L. Blawat: Platelet aggregation by Staphylococcal toxins. Thrombos. Diathes. haemorrh. (Stuttg.) 15, 69 (1965).Google Scholar
- Karnovsky, M. J.: Simple methods for “staining with lead” at high pH in electron microscopy. J. Cell Biol. 11, 729 (1961).Google Scholar
- Kielley, W. W.: Mitochondrial ATPase. In: Methods in enzymology. Vol. 2, p. 593, 1955.Google Scholar
- Libánská, J.: The influence of EDTA, trisodiumcitrate and heparin on the ultrastructure of blood platelets. Thrombos. Diathes. haemorrh. (Stuttg.) 16, 371 (1966).Google Scholar
- Lowry, O. H., N. J. Rosenbrough, A. L. Farr, and R. J. Randall: Protein measurement with Folin phenol reagent. J. biol. Chem. 193, 256 (1951).Google Scholar
- Mašek, K., and H. Rašková: Action of Staphylococcal α toxin on blood platelets. Čs. Physiol. 15, 404 (1966).Google Scholar
- -- -- unpublished observation (1968).Google Scholar
- —— ——, and E. Novák: The mechanism of Staphylococcal alpha toxin action on smooth muscle. Life Sci. 6, 1859 (1967).Google Scholar
- Nachman, R., A. Marcus, and D. Zucker-Franklin: Immunologic studies of proteins with subcellular fractions of normal and thrombosthenic platelets. Personal communication (1967).Google Scholar
- Prager, M. D., and M. A. Fletscher: The effect of enzymatic release of sialic acid from human erythrocytes on Rh agglutinations. J. Immunol. 97, 165 (1966).Google Scholar
- Salzman, E. W., D. A. Chambers, and L. L. Neri: Possible mechanism of aggregation of blood platelets by adenosine diphosphate. Nature (Lond.) 210, 167 (1966).Google Scholar
- Scott, R. B.: Activation of glycogenphosphorylase in blood platelets. Blood 30, 321 (1967).Google Scholar
- Siegel, I., and S. Cohen: Action of Staphylococcal toxin on human platelets. J. infect. Dis. 114, 488 (1964).Google Scholar
- Thal, A., and W. Egner: The site of action of Staphylococcal toxin. J. exp. Med. 113, 67 (1961).Google Scholar
- Warren, L.: The thiobarbituric acid assay of Sialic acids. J. biol. Chem. 234, 1917 (1959).Google Scholar
- Weissbach, H., and B. G. Redfield: Factors affecting the uptake of 5-HT by human platelets in an inorganic medium. J. biol. Chem. 235, 3287 (1960).Google Scholar
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