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Biophysics of structure and mechanism

, Volume 10, Issue 3, pp 121–128 | Cite as

Isoelectric characteristics and the secondary structure of some nucleic acids

  • G. V. Sherbet
  • M. S. Lakshmi
  • F. Cajone
Article

Abstract

The isoelectric characteristics of some nucleic acid preparations from rat liver have been examined. 10S and 4S RNA species and SV-DNA were found to have isoelectric points of 5.2, 6.0–6.7, and 4.35 respectively. The molecular charge ratios (net negative charge/nucleotide) were calculated. Using SV-DNA as a standard, these isoelectric characteristics and charge ratios have been interpreted as indicating that the 10S and 4S RNAs have 35 and 56% of the molecules involved in secondary structure.

Key words

Nucleic acids Isoelectric characteristics Secondary structure 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • G. V. Sherbet
    • 1
  • M. S. Lakshmi
    • 1
  • F. Cajone
    • 2
  1. 1.Cancer Research UnitUniversity of Newcastle upon Tyne, The Royal Victoria InfirmaryNewcastle upon TyneEngland
  2. 2.Istituto di Patologia Generale, Centro di Patologia del C.N.R.Universitá degli StudiMilanoItalia

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