Histochemistry

, Volume 87, Issue 2, pp 107–113

Light and electron microscopical immunocytochemistry of 5′-nucleotidase in rat cerebellum

  • S. W. Schoen
  • M. B. Graeber
  • M. Reddington
  • G. W. Kreutzberg
Article

DOI: 10.1007/BF00533394

Cite this article as:
Schoen, S.W., Graeber, M.B., Reddington, M. et al. Histochemistry (1987) 87: 107. doi:10.1007/BF00533394

Summary

5′-Nucleotidase in nervous tissue has so far not been localised at the ultrastructural level using immunocytochemical techniques. We have now applied monoclonal antibodies and a polyclonal antiserum raised against this ecto-enzyme and describe the distribution of 5′-nucleotidase antigenicity in rat cerebellum both at the light and electron microscopic levels. Within all cerebellar layers, 5′-nucleotidase immunoreactivity was found on plasma membranes of glial elements, i.e. Bergmann glial cell processes crossing the molecular layer, astrocytic end-feet around blood vessels and glial cell extensions surrounding single Purkinje cells. In the granular layer, 5′-nucleotidase immunoreactivity was present on glial membranes interposed between granule cells. Neuronal cells or processes were devoid of immunoreactivity. The immunocytochemical results were compared with conventional 5′-nucleotidase histochemistry. Both techniques showed the same ecto-localisation of the enzyme and favour the view of 5′-nucleotidase being predominantly situated at glial plasma membranes.

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • S. W. Schoen
    • 1
  • M. B. Graeber
    • 1
  • M. Reddington
    • 1
  • G. W. Kreutzberg
    • 1
  1. 1.Department of NeuromorphologyMax-Planck-Institute for PsychiatryMartinsriedGermany

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