Light and electron microscopical immunocytochemistry of 5′-nucleotidase in rat cerebellum
- Cite this article as:
- Schoen, S.W., Graeber, M.B., Reddington, M. et al. Histochemistry (1987) 87: 107. doi:10.1007/BF00533394
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5′-Nucleotidase in nervous tissue has so far not been localised at the ultrastructural level using immunocytochemical techniques. We have now applied monoclonal antibodies and a polyclonal antiserum raised against this ecto-enzyme and describe the distribution of 5′-nucleotidase antigenicity in rat cerebellum both at the light and electron microscopic levels. Within all cerebellar layers, 5′-nucleotidase immunoreactivity was found on plasma membranes of glial elements, i.e. Bergmann glial cell processes crossing the molecular layer, astrocytic end-feet around blood vessels and glial cell extensions surrounding single Purkinje cells. In the granular layer, 5′-nucleotidase immunoreactivity was present on glial membranes interposed between granule cells. Neuronal cells or processes were devoid of immunoreactivity. The immunocytochemical results were compared with conventional 5′-nucleotidase histochemistry. Both techniques showed the same ecto-localisation of the enzyme and favour the view of 5′-nucleotidase being predominantly situated at glial plasma membranes.