Foam behavior of biological media

I. Protein foams
  • K. Kalischewski
  • W. Bumbullis
  • K. Schügerl
Biotechnology

Summary

The foaminess and the time dependence of the surface tension σ of aqueous bovine serum albumin, bacterium protease and amyloglucosidase solutions were estimated and the time tDG necessary for them to attain their equilibrium surface tensions was determined. The foaminess could be described as a simple function of tDG. The time dependence of σ could be evaluated by Eq. (1)
$$\begin{gathered}{\text{ }}1{\text{n [1/(1 - y)] = b t}}^{\text{n}} \hfill \\{\text{where y = (}}\sigma _{\text{O}} {\text{ - }}\sigma _{{\text{s,t}}} {\text{)/(}}\sigma _{\text{O}} {\text{ - }}\sigma _{{\text{s,}}\infty } {\text{),}} \hfill \\{\text{ }}\sigma _{\text{O}} {\text{ = surface tension of pure solvent}} \hfill \\{\text{ }}\sigma _{{\text{s,t}}} {\text{ = surface tension of protein solution at time t}} \hfill \\{\text{ }}\sigma _{{\text{s,}}\infty } {\text{ = equilibrium surface tension of solution}}{\text{. }} \hfill \\\end{gathered}$$
(1)
The constant n of Eq. (1) is dependent on the protein concentration as well as on b. The foaminess can also be described as a simple function of n and/or nob.

Keywords

Albumin Foam Bovine Serum Bovine Serum Albumin Surface Tension 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Symbols

b

constant in Eq. (4)

C

concentration of the protein

n

constant in Eq. (4)

T

temperature

t

time (measured from the beginning of the determination of the surface tension σ)

tDG

time, necessary to attain the equilibrium surface tension σequil

V

O − σs,∞)/(σs,t − σs,∞)

Vs

equilibrium volume of the foam

Vtg

volumetric gas flow rate during the estimation of Σ

Σ

foaminess

σ

surface tension

AMG

amyloglucosidase

BP

bacterium protease

BSA

bovine serum albumin

CMC

critical micelle concentration

mN/n

milli Newton meter−1

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References

  1. Avrami, M. (1939). J. Chem. Phys. 7, 1103Google Scholar
  2. Avrami, M. (1940). J. Chem. Phys. 8, 212Google Scholar
  3. Avrami, M. (1941). J. Chem. Phys. 9, 177Google Scholar
  4. Benjamins, J., Fejter, J.A., Evans, M.T.A., Graham, E.E., Phillips, M.C. (1975). Faraday Discuss. Chem. Soc. 59, 218Google Scholar
  5. Bikermann, J. (1938). Trans. Faraday Soc. 34, 634Google Scholar
  6. Cumper, C.W.N., Alexander, A.E. (1950). Trans. Faraday Soc. 46, 235Google Scholar
  7. Cumper, C.W.N. (1953). Trans Faraday Soc. 49, 1360Google Scholar
  8. Defay, R., Prigogine, I., Bellemans, A., Everett, D.H. (1966). Surface Tension and Adsorption Capt. 13, LondonGoogle Scholar
  9. Harkins, W.D., Jordan, H.F. (1930). J. Am. Chem. Soc. 52, 1751Google Scholar
  10. Schay, G. (1969). In: Surface and Colloid Science, E. Matijevic, ed., vol. 2, p. 155, New York: Wiley InterscienceGoogle Scholar
  11. Ueberreiter, K., Moritomo, S., Steulman, R. (1974). Coll. + Polym. 252, 273Google Scholar
  12. Ward, A.F.H., Tordai, L. (1946). J. Chem. Phys. 14, 452Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • K. Kalischewski
    • 1
  • W. Bumbullis
    • 1
  • K. Schügerl
    • 1
  1. 1.Institut für Technische Chemie der Universität HannoverHannover 1Federal Republic of Germany

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