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Archives of Microbiology

, Volume 133, Issue 4, pp 257–260 | Cite as

Bacterial scavenging: Utilization of fatty acids localized at a solid-liquid interface

  • B. Kefford
  • S. Kjelleberg
  • K. C. Marshall
Original Papers

Abstract

A model oligotrophic aquatic system involving localization of fatty acids on a solid surface was used to quantitate scavenging by three bacteria; Leptospira biflexa patoc 1 which adheres reversibly, pigmented Serratia marcescens EF190 which adheres irreversibly, and a non-pigmented hydrophilic mutant of EF190. The Leptospira and pigmented Serratia displayed two distinct scavenging strategies which are related to their different methods of adhesion. The Leptospira efficiently scavenged [1-14C] stearic acid from the surface in 24 h, whereas the pigmented hydrophobic Serratia initially showed a faster rate of removal but the overall rate was considerably slower than that of the Leptospira. The hydrophilic, non-pigmented Serratia required 50h incubation to remove significant amounts of the labelled fatty acid. The greater scavenging ability of the hydrophobic pigmented Serratia strain compared to the hydrophilic non-pigmented mutant could not be attributed to differences in viability of fatty acid metabolism. The hydrophobicity of the pigmented Serratia allows for firmer adhesion and greater interaction with the surface localized nutrients.

Key words

Bacterial scavenging Surface localized nutrients Leptospira Serratia Hydrophobic interactions 

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References

  1. Bitton G, Marshall KC (1980) Adsorption of microorganisms to surfaces. Wiley-Interscience, New YorkGoogle Scholar
  2. Blanchard DC, Syzdek LD (1978) Seven problems in bubble and jet drop researches. Limnol Oceanogr 23:389–400Google Scholar
  3. Brown CM, Ellwood DC, Hunter JR (1977) Growth of bacteria at surfaces: influence of nutrient limitation. FEMS Microbiol Lett 1:163–166Google Scholar
  4. Cox PJ, Twigg GI (1974) Leptospiral motility. Nature 250:260–261Google Scholar
  5. Ellwood DC, Melling J, Rutter P (1979) Adhesion of microorganisms to surfaces. Academic Press, New YorkGoogle Scholar
  6. Fletcher M (1979) A microautoradiographic study of the activity of attached and free-living bacteria. Arch Microbiol 122:271–274Google Scholar
  7. Goulder R (1977) Attached and free bacteria in an estuary with abundant suspended solids. J Appl Bacteriol 43:399–405Google Scholar
  8. Harvey RW, Young LY (1980) Enumeration of particle-bound and unattached respiring bacteria in the salt marsh environment. Appl Environ Microbiol 40:156–160Google Scholar
  9. Helprin JJ, Hiatt CW (1957) The effect of fatty acids on the respiration of Leptospira icterohemorrhagiae. J Infect Dis 100:136–140Google Scholar
  10. Hendricks CW (1974) Sorption of heterotrophic and enteric bacteria to glass surfaces in the continuous culture of river water. Appl Microbiol 28:572–578Google Scholar
  11. Hermansson M, Kjelleberg S, Norkrans B (1979) Interaction of pigmented wild type and pigmentless mutant of Serratia marcescens with lipid surface film. FEMS Microbiol Lett 6:129–132Google Scholar
  12. Jannasch HW, Pritchard PH (1972) The role of inert particulate matter in the activity of aquatic micoorganisms, Mem 1st Ital Idrobiol 29 Suppl: 289–308Google Scholar
  13. Kirchman D, Mitchell R (1982) Contribution of particle-bound bacteria to total microheterotrophic activity in five pounds and two marshes. Appl Environ Microbiol 43:200–209Google Scholar
  14. Kjelleberg S, Lagercrantz C, Larsson T (1980) Quantitative analysis of bacterial hydrophobicity studies by the binding of dodecanoic acid. FEMS Microbiol Lett 7:41–44Google Scholar
  15. Lewin RA, Lounsbery DM (1969) Isolation cultivation and characteristics of flexibacteria. J Gen Microbiol 58:145–170Google Scholar
  16. Marshall KC (1976) Interfaces in microbial ecology. Harvard University Press CambridgeGoogle Scholar
  17. Matin A, Veldkamp H (1978) Physiological basis of the selective advantage of Spirillum sp. in a carbon-limited environment. J Gen Microbiol 105:187–197Google Scholar
  18. Miles AA, Misra SS (1938) Estimation of bactericidal power of blood. J Hyg 38: 732–748Google Scholar
  19. Minato H, Suto T (1976) Technique for fractionation of bacteria in rumen microbial ecosystem. I. Attachment of rumen bacteria to starch granules and elution of bacteria attached to them. J Gen Appl Microbiol 22:259–276Google Scholar
  20. Purkayastha M, Williams RP (1960) Association of pigment with the cell envelope of Serratia marcescens (Chromobacterium prodigiosum). Nature 187:349–350Google Scholar
  21. Stark WH, Stadler J, McCoy E (1938) Some factors affecting the bacterial population of fresh water lakes. J Bacteriol 36:653–654Google Scholar
  22. Takakuwa S, Fujimori T, Iwasaki H (1979) Some properties of cell-sulfur adhesion in Thiobacillus thiooxidans. J Gen Appl Microbiol 25: 21–29Google Scholar
  23. Williams RP (1973) Biosynthesis of prodigiosin, a secondary metabolite of Serratia marcescens. Appl Microbiol 25:396–402Google Scholar
  24. ZoBell CE, Anderson DQ (1936) Observations on the multiplication of bacteria in different volumes of sea water and the influence of solid surfaces. Biol Bull Woods Hole 71:324–342Google Scholar
  25. Zvyagintseva IS, Zvyagintsev DG (1969) Effect of microbial cell adsorption onto steroid crystals on the transformation of the steroid. Microbiology 38:691–694Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • B. Kefford
    • 1
  • S. Kjelleberg
    • 1
  • K. C. Marshall
    • 1
  1. 1.The University of New South WalesKensingtonAustralia

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