Biochemical Genetics

, Volume 15, Issue 5–6, pp 509–519 | Cite as

Genetic control of UDPglucose:flavonol 3-O-glucosyltransferase in the endosperm of maize

  • H. K. Dooner
  • O. E. Nelson


The enzyme UDPglucose:flavonol 3-O-glucosyltransferase is shown to be under the coordinate control of three genes involved in anthocyanin biosynthesis in the aleurone of maize: C, R, and Bz. Of the three, Bz appears to be the structural gene. Data presented here (dosage comparisons, induction in the mutant c-p, and effect of paramutation at R) indicate that the enzyme is inducible by substances resulting from the action of the C and R genes and that active forms of C and R are required for this induction. Mechanisms of regulation of the Bz gene by C and R are discussed.

Key words

anthocyanin biosynthesis flavonoid maize pigment genes UDPG flavonol glucosyltransferase 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brink, R. A. (1956). A genetic change associated with the R locus in maize which is directed and potentially reversible. Genetics 41872.Google Scholar
  2. Brink, R. A. (1973). Paramutation. Ann. Rev. Genet. 7129.Google Scholar
  3. Britten, R. J., and Davidson, E. H. (1969). Gene regulation for higher cells: A theory. Science 165349.Google Scholar
  4. Chen, S.-M., and Coe, E. H., Jr. (1977). Control of anthocyanin synthesis by the C locus in maize. Biochem. Genet. 15333.Google Scholar
  5. Coe, E. H. (1962). Spontaneous mutation of the aleurone color inhibitor in maize. Genetics 47779.Google Scholar
  6. Dooner, H. K., and Kermicle, J. L. (1976). Displaced and tandem duplications in the long arm of chromosome 10 in maize. Genetics 82309.Google Scholar
  7. Emerson, R. A. (1921). The genetic relations of plant colors in maize. Cornell Univ. Agr. Exp. Sta. Mem. 391.Google Scholar
  8. Fincham, J. R. S., and Sastry, G. R. K. (1974). Controlling elements in maize. Ann. Rev. Genet. 815.Google Scholar
  9. Grisebach, H. (1965). Biosynthesis of flavonoids. In Goodwin, T. W. (ed.), Chemistry and Biochemistry of Plant Pigments, Academic Press, New York, pp. 279–308.Google Scholar
  10. Grisebach, H., and Hahlbrock, K. (1974). Enzymology and regulation of flavonoid and lignin biosynthesis in plants and plant cell suspension cultures. Recent Adv. Phytochem. 821.Google Scholar
  11. Harborne, J. B. (1967). Comparative Biochemistry of the Flavonoids, Academic Press, New York.Google Scholar
  12. Kermicle, J. L. (1970). Dependence of the R-mottled aleurone phenotype in maize on mode of sexual transmission. Genetics 6669.Google Scholar
  13. Kirby, L. T., and Styles, E. D. (1970). Flavonoids associated with specific gene action in maize aleurone and the role of light in substituting for the action of a gene. Can. J. Genet. Cytol. 12934.Google Scholar
  14. Larson, R. L. (1971). Glucosylation of quercetin by a maize pollen enzyme. Phytochemistry 103073.Google Scholar
  15. Larson, R. L., and Coe, E. H. (1968). Enzymatic action of the Bz anthocyanin factor in maize. Proc. XII Inst. Congr. Genet. 1131 (abst.).Google Scholar
  16. Larson, R. L., and Coe, E. H., Jr. (1977). Gene-dependent flavonoid glucosyltransferase in maize. Biochem. Genet. 15153.Google Scholar
  17. Larson, R. L., and Lonergan, C. M. (1972). Glucosyltransferase activity in a water extract of maize pollen. Planta (Berl.) 103361.Google Scholar
  18. Larson, R. L., and Lonergan, C. M. (1973). Flavonoid glucosyltransferase in maize. Cereal Res. Commun. 113.Google Scholar
  19. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193265.Google Scholar
  20. Neuffer, M. G., Jones, L., and Zuber, M. S. (1968). The Mutants of Maize, Crop Science Society of America, Madison, Wis.Google Scholar
  21. Potty, V. H. (1969). Determination of protein in the presence of phenols and pectins. Anal. Biochem. 29535.Google Scholar
  22. Reddy, A. R., and Reddy, G. M. (1971). Chemico-genetic studies of leucoanthocyanidin in a maize mutant. Curr. Sci. 40335.Google Scholar
  23. Reddy, G. M. (1964). Genetic control of leucoanthocyanidin formation in maize. Genetics 50485.Google Scholar
  24. Reddy, G. M., and Coe, E. H. (1962). Inter-tissue complementation: A simple technique for direct analysis of gene-action sequence. Science 138149.Google Scholar
  25. Robertson, D. S. (1955). The genetics of vivipary in maize. Genetics 40745.Google Scholar
  26. Sutter, A., and Grisebach, H. (1973). UDP-glucose: Flavonol 3-O-glucosyltransferase from cell suspension cultures of parsley. Biochim. Biophys. Acta 309289.Google Scholar
  27. van Sumere, C. F., Cottenie, J., de Greef, J., and Kint, J. (1972). Biochemical studies in relation to the possible germination regulatory role of naturally occurring coumarin and phenolics. Recent Adv. Phytochem. 4165.Google Scholar

Copyright information

© Plenum Publishing Corp. 1977

Authors and Affiliations

  • H. K. Dooner
    • 1
  • O. E. Nelson
    • 1
  1. 1.Department of GeneticsUniversity of WisconsinMadison

Personalised recommendations