Current Genetics

, Volume 28, Issue 6, pp 512–516 | Cite as

Identification and characterization of CEN12 in the budding yeast Saccharomyces cerevisiae

  • Alison E. Gammie
  • Mark D. Rose
Original Paper
Received:

Abstract

In this paper we report the cloning, sequencing and functional characterization of CEN12 and an associated autonomously replicating sequence (ARS) from the budding yeast Saccharomyces cerevisiae. In the course of studying a dynamin-related gene, DNM1, we previously physically mapped the gene to chromosome 12. Genetic mapping showed that the gene was tightly linked (0.35 cM) to the centromere. Subcloning experiments revealed that a centromerelike activity was included in a small segment of DNA immediately downstream from the DNM1 gene. Mitotic centromere activity was discerned by the ability of the region to de-stabilize a centromere-containing plasmid, and to stabilize an ARS-containing plasmid. Meiotic centromere activity was determined by the first-division segregation in crosses of ARS plasmids containing this region. The DNA sequence of this region revealed a sequence with strong homology to the consensus for yeast centromeres.

Key words

Yeast Saccharomyces Centromere Autonomously replicating segments 
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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Alison E. Gammie
    • 1
  • Mark D. Rose
    • 1
  1. 1.Department of Molecular BiologyPrinceton UniversityPrincetonUSA

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