Metabolic products of microorganisms, 165th communication

Isolation and quantitative determination of siderochromes
  • H. -P. Fielder
  • J. Sauerbier
Industrial microbiology


Isolation of siderochromes from culture filtrates by extraction with a mixture of chloroform and phenol was replaced by adsorption chromatography with Amberlite XAD-2. For the antibiotic albomycin, the purification was possible by ion-exchange chromatography with SP-Sephadex, and for the sideramine ferricrocin by exclusion chromatography with Bio-Gel P-2. Quantitation of albomycin was done by an agar plate diffusion test, and of ferricrocin by high-pressure liquid chromatography and photometric determination. Thinlayer and high-pressure liquid chromatographic systems were developed for checking homogeneity.


Chromatography Phenol Culture Filtrate Chromatographic System Metabolic Product 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • H. -P. Fielder
    • 1
  • J. Sauerbier
    • 1
  1. 1.Institut für Biologie IILehrstuhl Mikrobiologie I der UniversitätTübingen 1Federal Republic of Germany

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