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Metabolic products of microorganisms, 165th communication

Isolation and quantitative determination of siderochromes
  • H. -P. Fielder
  • J. Sauerbier
Industrial microbiology

Summary

Isolation of siderochromes from culture filtrates by extraction with a mixture of chloroform and phenol was replaced by adsorption chromatography with Amberlite XAD-2. For the antibiotic albomycin, the purification was possible by ion-exchange chromatography with SP-Sephadex, and for the sideramine ferricrocin by exclusion chromatography with Bio-Gel P-2. Quantitation of albomycin was done by an agar plate diffusion test, and of ferricrocin by high-pressure liquid chromatography and photometric determination. Thinlayer and high-pressure liquid chromatographic systems were developed for checking homogeneity.

Keywords

Chromatography Phenol Culture Filtrate Chromatographic System Metabolic Product 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bickel, H., Gäumann, E., Keller-Schierlein, W., Prelog, V., Vischer, E., Wettstein, A., Zähner, H. (1960). Experientia 16, 129–133Google Scholar
  2. Braznikova, M.G., Mikeš, O., Lomakina, N.N. (1957). Biochimija 22, 111–117Google Scholar
  3. Davis, B.D., Mingioli, E.S. (1950). J. Bacteriol. 60, 17–28Google Scholar
  4. Kappner, M., Hasenböhler, A., Zähner, H. (1977). Arch. Microbiol. 115, 323–331Google Scholar
  5. Keller-Schierlein, W., Prelog, V., Zähner, H. (1964). Fortschr. Chem. Org. Naturst. 22, 279–322Google Scholar
  6. Maehr, H., Berger, J. (1969). Biotechnol. Bioeng. 11, 1111–1123Google Scholar
  7. Maehr, H., Pitcher, R.G. (1971). J. Antibiot. 24, 830–834Google Scholar
  8. Snyder, L.R., Kirkland, J.J. (1974). Introduction to modern liquid chromatography. New York, London, Sydney, Toronto: John Wiley & SonsGoogle Scholar
  9. Thrum, H. (1957). Naturwiss. 44, 561–562Google Scholar
  10. Zähner, H., Keller-Schierlein, W., Hütter, R., Hess-Leisinger, K., Deér, A. (1963). Arch. Microbiol. 45, 119–135Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • H. -P. Fielder
    • 1
  • J. Sauerbier
    • 1
  1. 1.Institut für Biologie IILehrstuhl Mikrobiologie I der UniversitätTübingen 1Federal Republic of Germany

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