Archives of Dermatological Research

, Volume 281, Issue 7, pp 454–462

Adhesion molecule mapping in normal human skin

  • U. Konter
  • I. Kellner
  • E. Klein
  • R. Kaufmann
  • V. Mielke
  • W. Sterry
Original Contributions

Summary

Adhesion molecules are a rapidly growing group of cell surface receptors providing cell-cell and cell-matrix interactions. Their physiological role in tissue homeostasis as well as cellular migration and differentiation is increasingly appreciated. In the present study we have analyzed the expression pattern of most adhesion molecules of the integrin family as well as of adhesion molecules belonging to the immunoglobulin superfamily in normal human skin. We provide evidence that expression of adhesion molecules in the various cutaneous cell systems follows a constant distribution. Moreover, the physiological mononuclear infiltrate of the skin also expresses a variety of adhesion molecules enabeling these cells to migrate or to reside within the skin. Furthermore, our results indicate that intercellular adhesion molecule-1 is not a prerequisite for lymphocyte epidermotropism as frequently stated. Our data provide a rational basis to analyze changing adhesion molecule expression in inflammatory and neoplastic skin diseases.

Key words

Adhesion molecules Integrins Lymphocyte epidermotropism 

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References

  1. 1.
    Amiot P, Gaudebout H, Dastot M, Schmid M, Boumsell L (1989) Study of the relationships between CDw29, CD31, and GMP140 mAb on hematopoietic cells. Tissue Antigens 33:354Google Scholar
  2. 2.
    Argraves WS, Suzuki S, Arai H, Thompson K, Pierschbacher MD, Ruoslahti E (1987) Amino acid sequence of the fibronectin receptor. J Cell Biol 105:1183–1190Google Scholar
  3. 3.
    Bierer BE, Barbosa J, Herrmann S, Burakoff S (1988) Interaction of CD2 with its ligand, LFA-3, in human T cell proliferation. J Immunol 140:3358–3363Google Scholar
  4. 4.
    Bos JD, Zonneveld I, Das PK, Krieg SR, Van der Loos CM, Kapsenberg ML (1987) The skin immune system (SIS): distribution and immunophenotype of lymphocyte subpopulations in normal human skin. J Invest Dermatol 88:569–573Google Scholar
  5. 5.
    Bullock GR, Petrusz P (1982) Techniques in immunocytochemistry. Academic Press, New YorkGoogle Scholar
  6. 6.
    Davignon D, Martz E, Reynolds T (1981) Monoclonal antibody to a novel lymphocyte function-associated antigen (LFA-1): mechanism of blocking of T lymphocyte-mediated killing and effects on other T and B lymphocyte functions. J Immunol 127:590–595Google Scholar
  7. 7.
    Duijvestijn A, Hamann A (1989) Mechanisms and regulation of lymphocyte migration. Immunol Today 10:23–28Google Scholar
  8. 8.
    Dustin ML, Springer TA (1988) Lymphocyte function associated antigen 1 (LFA-1) interaction with intercellular adhesion molecule 1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells. J Biol 107:321–331Google Scholar
  9. 9.
    Dustin ML, Rothlein R, Bahn AK, Dinarello CA (1986) Induction by IL-1 and interferon-τ: tissue distribution, biochemistry and function of a natural adherence molecule (ICAM-1). J Immunol 137:245–254Google Scholar
  10. 10.
    Dustin ML, Sanders ME, Shaw S, Springer TA (1987) Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion. J Exp Med 165:677–692Google Scholar
  11. 11.
    Dustin ML, Singer KH, Tuck DT, Springer TA (1988) Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon-τ and is mediated by intercellular adhesion molecule 1 (ICAM-1). J Exp Med 167:1323–1340Google Scholar
  12. 12.
    Dustin ML, Staunton DE, Springer TA (1988) Supergene families meet in the immune system. Immunol Today 9:213–215Google Scholar
  13. 13.
    Elder JT, Fisher GJ, Lindquist PB, Bennett GL, Pittelkow MR, Coffey RJ, Ellingsworth L, Derynck R, Voorhees JJ (1989) Overexpression of transforming growth factor α in psoriatic epidermis. Science 243:811–814Google Scholar
  14. 14.
    Feller AC, Parwaresch MR, Wacker H-H, Radzun H-J, Lennert K (1983) Combined immunohistochemical staining for surface IgD and T-lymphocyte subsets with monoclonal antibodies in human tonsils. Histochemical J 15:557–562Google Scholar
  15. 15.
    Freyer DR, Morganroth ML, Rogers CE, Arnaout MA, Todd RF (1988) Modulation of surface CD11/CD18 glycoproteins (Mol, LFA-1, p150, 95) by human mononuclear phagocytes. Clin Immunol Immunopathol 46:272–283Google Scholar
  16. 16.
    Haskard D, Cavender D, Beatty P, Springer TA, Ziff M (1986) T lymphocyte adhesion to endothelial cells: mechanisms demonstrated by anti-LFA-1 monoclonal antibodies. J Immunol 137:2901–2906Google Scholar
  17. 17.
    Heino J, Ignotz RA, Hemler ME, Crouse C, Massagué J (1988) Regulation of cell adhesion receptors by transforming growth factor-β. J Biol Chem 264:380–388Google Scholar
  18. 18.
    Hemler ME (1988) Adhesive protein receptors on hematopoietic cells. Immunol Today 9:109–113Google Scholar
  19. 19.
    Hemler ME, Jacobson JG (1987) Cell matrix adhesion-related proteins VLA-1 and VLA-2: regulation of expression on T cells. J Immunol 138:2941–2948Google Scholar
  20. 20.
    Hemler ME, Sachez-Madrid F, Flotte TJ, Krensky AM, Burakoff SJ, Bhan AK, Springer TA, Strominger JL (1984) Glycoproteins of 210,000 and 130,000 M. W. on activated T cells: cell distribution and antigenic relation to components on resting cells and cell lines. J Immunol 132:3011–3018Google Scholar
  21. 21.
    Hemler ME, Jacobson JG, Brenner MB, Mann D, Strominger JL (1985) V1A-1: a T cell antigen which defines a novel late stage of human T cell activation. Eur J Immunol 15:502–508Google Scholar
  22. 22.
    Hemler ME, Huang C, Schwarz L (1987) The VLA protein family. Characterization of five distinct cell surface heterodimers each with a common 130,000 molecular weight β subunit. J Biol Chem 262:3300–3309Google Scholar
  23. 23.
    Hemler ME, Huang C, Takada Y, Schwarz L, Strominger JL, Clabby ML (1987) Characterization of the cell surface heterodimer VLA-4 and related peptides. J Biol Chem 262:11478–11485Google Scholar
  24. 24.
    Hemler ME, Crouse C, Takada Y, Sonnenberg A (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures. J Biol Chem 263:7660–7665Google Scholar
  25. 25.
    Hynes RO (1987) Integrins: a family of cell surface receptors. Cell 48:549–554Google Scholar
  26. 26.
    Johnson JP, Stade BG, Holzmann B, Schwäble W, Riethmüller G (1989) De novo expression of intercellular-adhesion molecule-1 in melanoma correlates with increased risk of metastasis. Proc Natl Acad Sci USA 86:641–644Google Scholar
  27. 27.
    Klein CE, Cordon-Cardo C, Soehnchen R, Cote R, Oettgen HF, Eisinger M, Old L (1987) Changes in cell surface glycoprotein expression during differentiation of human keratinocytes. J Invest Dermatol 89:500–506Google Scholar
  28. 28.
    Kramer RH, McDonald KA, Crowley E, Ramos DM, Damsky CH (1989) Melanoma cell adhesion to basement membrane mediated by integrin-related complexes. Cancer Res 49:393–402Google Scholar
  29. 29.
    Kürzinger K, Reynolds T, Germain RN, Davignon D, Martz E, Springer TA (1981) A novel lymphocyte function-associated antigen (LFA-1): cellular distribution, quantitative expression and structure. J Immunol 127:596–602Google Scholar
  30. 30.
    Makgoba MW, Sanders ME, Luce GEG (1988) Functional evidence that intercellular adhesion molecule-1 (ICAM-1) is a ligand for LFA-1-dependent adhesion in T cell-mediated cytotoxity. Eur J Immunol 18:637–640Google Scholar
  31. 31.
    Marlin SD, Springer TA (1987) Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell 51:813–819Google Scholar
  32. 32.
    Marx JL (1989) New family of adhesion proteins discovered. Science 243:1144Google Scholar
  33. 33.
    Miossec P, Cavender D, Ziff M (1986) Production of interleukin 1 by human endothelial cells. J Immunol 136:2486–2491Google Scholar
  34. 34.
    Modderman PW, Von dem Borne AEG Kr, Sonneberg A (1989) VLA-2, VLA-5, VLA-6 function as activation-independent platelet adhesion receptors for collagen, fibronectin and laminin, respectively. Tissue Antigens 33:357Google Scholar
  35. 35.
    Nickoloff BJ (1988) Role of interferon-μ in cutaneous trafficking of lymphocytes with emphasis on molecular and cellular adhesion events. Arch Dermatol 124:1835–1842Google Scholar
  36. 36.
    Nickoloff BJ, Reusch MK, Bensch K, Karasek MA (1988) Preferential binding of monocytes and Leu 2+ T lymphocytes to interferon-gamma treated cultured skin endothelial cells and keratinocytes. Arch Dermatol Res 280:235–245Google Scholar
  37. 37.
    Pischel KD, Hemler ME, Huang C, Bluestein HG, Woods VL (1987) Use of the monoclonal antibody 12F1 to characterize the differentiation antigen VLA-2. J Immunol 138:226–233Google Scholar
  38. 38.
    Pischel KD, Bluestein HG, Woods Jr VL (1988) Platelet glycoproteins Ia, Ic, and IIa are physicochemically indistinguishable from the very late activation antigens adhesionrelated proteins of lymphocytes and other cell types. J Clin Invest 81:505–513Google Scholar
  39. 39.
    Ruoslahti E (1988) Fibronectin and its receptors. Ann Rev Biochem 57:375–413Google Scholar
  40. 40.
    Ruoslahti E, Pierschbacher MD (1987) New perspectives in cell adhesion: RGD and integrins. Science 238:491–497Google Scholar
  41. 41.
    Sánchez-Madrid F, De Landázuri MO, Morago G, Cebrián M, Acevedo A, Bernabeu C (1986) VLA-3: a novel polypeptide association within the VLA molecular complex: cell distribution and biochemical characterization. Eur J Immunol 16:1343–1349Google Scholar
  42. 42.
    Sanders ME, Makgoba MW, Sharrow SO, Stephany D, Springer TA, Young HO, Shaw S (1988) Human memory T lymphocytes express increased levels of three adhesion molecules (LFA-3, CD2 and LFA-1) and three other molecules (UCHL-1, CDw29 and Pgp-1) and have enhanced INF-μ production. J Immunol 140:1401–1407Google Scholar
  43. 43.
    Sanders ME, Malegapuru W, Makgoba M, Shaw S (1988) Human naive and memory T cells: reinterpretation of helper and suppressor-inducer subsets. Immunol Today 9:195–198Google Scholar
  44. 44.
    Selvaraj P, Plunkett ML, Dustin M, Sanders ME, Shaw S, Springer TA (1987) The T lymphocyte glycoprotein CD2 binds the cell surface ligand LFA-3. Nature 326:400–403Google Scholar
  45. 45.
    Smolle J (1988) Mononuclear cell patterns in the skin. An immunohistological and morphometrical analysis. Am J Dermatopathol 10:36–46Google Scholar
  46. 46.
    Smolle J, Ehall R, Kerl H (1985) Inflammatory cell types in normal human epidermis — an immunohistochemical and morphometric study. Acta Derm Venereol (Stockh) 65:479–483Google Scholar
  47. 47.
    Sonnenberg A, Janssen H, Hogervorst F, Calafat J, Hilgers J (1987) A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody. J Biol Chem 262:10376–10383Google Scholar
  48. 48.
    Springer TA, Anderson DC (1986) The importance of the Mac-1, LFA-1 glycoprotein family in monocyte and granulocyte adherence, chemotaxis, and migration into inflammatory sites: insights from an experiment of nature. Biochemistry of macrophages. Ciba Foundation Symp 118:102–126Google Scholar
  49. 49.
    Springer TA, Dustin ML, Kishimoto TK, Marlin SD (1987) The lymphocyte function-associated LFA-1, CD2 and LFA-3 molecules: cell adhesion receptors of the immune system. Annu Rev Immunol 5:223–252Google Scholar
  50. 50.
    Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer ZA (1988) Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrine supergene families. Cell 52:925–933Google Scholar
  51. 51.
    Takada Y, Huang C, Hemler ME (1987) Fibronectin receptor structures in the VLA family of heterodimers. Nature 326:607–609Google Scholar
  52. 52.
    Toda K-I, Grinnell F (1987) J Invest Dermatol 88:412–417Google Scholar
  53. 53.
    Terranova VP, Williams JE, Liotta LA, Martin GR (1984) Modulation of the metastatic activity of melanoma cells by laminin and fibronectin. Science 226:982–985Google Scholar
  54. 54.
    Wallner BP, Frey AZ, Tizard R, Mattaliano R, Hession C, Sanders ME, Dustin ML, Springer TA (1987) Primary structure of lymphocyte function-associated antigen 3 (LFA-3). The ligand of the T lymphocyte CD2 glycoprotein. J Exp Med 166:923–932Google Scholar
  55. 55.
    Williams AF, Barclay AN (1988) The immunoglobulin superfamily — domains for cell surface recognition. Annu Rev Immunol 6:381–405Google Scholar
  56. 56.
    Wright SD, Craigmyle LS, Silverstein S (1983) Fibronectin and serum amyloid P component stimulate C3b-and C3bi-mediated phagocytosis in cultured human monocytes. J Exp Med 158:1338–1343Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • U. Konter
    • 1
  • I. Kellner
    • 1
  • E. Klein
    • 2
  • R. Kaufmann
    • 2
  • V. Mielke
    • 1
  • W. Sterry
    • 1
  1. 1.Department of DermatologyUniversity of Kiel, KielKiel 1Germany
  2. 2.Department of DermatologyUniversity of UlmUlmGermany

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