Immuno gold staining (IGS) and immuno gold silver staining (IGSS) for the identification of the plant pathogenic bacterium Erwinia amylovora (Burrill) Winslow et al.
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- Van Laere, O., De Wael, L. & De Mey, J. Histochemistry (1985) 83: 397. doi:10.1007/BF00509198
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For the identification of the plant pathogenic bacterium Erwinia amylovora, the immuno gold staining (IGS) and immuno gold silver staining (IGSS) techniques are tested.
The IGS and IGSS methods are at least as sensitive an indirect immunofluorescence and require less primary antiserum. Moreover they have the advantage that the prepatrations can be conserved permanently and unchanged.
The preparation of the IGS can be observed with transmitted light or — with considerable better result — using epipolarization microscopy.
The IGSS method deserves special attention because of its high contrast in normal brigth field microscopy with transmitted light.