Histochemistry

, Volume 83, Issue 5, pp 397–399

Immuno gold staining (IGS) and immuno gold silver staining (IGSS) for the identification of the plant pathogenic bacterium Erwinia amylovora (Burrill) Winslow et al.

  • O. Van Laere
  • L. De Wael
  • J. De Mey
Article

DOI: 10.1007/BF00509198

Cite this article as:
Van Laere, O., De Wael, L. & De Mey, J. Histochemistry (1985) 83: 397. doi:10.1007/BF00509198

Summary

For the identification of the plant pathogenic bacterium Erwinia amylovora, the immuno gold staining (IGS) and immuno gold silver staining (IGSS) techniques are tested.

The IGS and IGSS methods are at least as sensitive an indirect immunofluorescence and require less primary antiserum. Moreover they have the advantage that the prepatrations can be conserved permanently and unchanged.

The preparation of the IGS can be observed with transmitted light or — with considerable better result — using epipolarization microscopy.

The IGSS method deserves special attention because of its high contrast in normal brigth field microscopy with transmitted light.

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • O. Van Laere
    • 1
  • L. De Wael
    • 1
  • J. De Mey
    • 2
  1. 1.State Research Station for Nematology and Entomology (C.L.O. Gent)MerelbekeBelgium
  2. 2.Janssen PharmaceuticaBeerseBelgium

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